Physiochemical and biological properties of modified collagen sponge from porcine skin
Physiochemical and biological properties of modified collagen sponge from porcine skin
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DOI：
10.1007/s11595-009-4619-2
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
Yuanyuan Xu;Jiming Wu;J. Guan;Xi-zheng Zhang;Zhi-hong Li;Pengfei Wang;Ruixin Li;Yong Guo
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文献类型：
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作者：
Yuanyuan Xu;Jiming Wu;J. Guan;Xi-zheng Zhang;Zhi-hong Li;Pengfei Wang;Ruixin Li;Yong Guo
本研究的目的是比较一步法 EDC/NHS 交联（EDC/NHS 组）和一步同时法 EDC/NHS 交联和肝素固定（EDC/NHS-肝素组）在改善天然胶原海绵（对照组）的理化和生物学特性方面的效果。改性胶原蛋白海绵克服了天然胶原蛋白海绵的缺点。红外光谱表明官能团的变化。 DSC数据表明EDC/NHS组热量转化的稳定性略高于EDC/NHS-Heparin组。 EDC/NHS-Heparin组的交联度、酶稳定性、形态稳定性和生物力学性质均高于EDC/NHS组，而EDC/NHS-Heparin组的水结合能力低于EDC/NHS组。 EDC/NHS-肝素组支架中的HUVEC增殖快、迁移好、分布均匀。一步同时法在上述方面取得了较好的效果，肝素化胶原基质增加了血管生成潜力，适合缺损修复和组织工程。
The aim of the present study was to compare one-step method to EDC/NHS crosslinking (EDC/NHS group) and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization (EDC/NHS- Heparin group) in improving physiochemical and biological properties of native collagen sponge (Control group). Modified collagen sponge overcome the disadvantages of native collagen sponge. IR spectra suggest the change of the functional groups. DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group. The crosslinking degree, stability against enzymes, stability in morphologically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group, whereas, the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group. HUVECs in EDC/NHS-Heparin group scaffold proliferate fast, migrate well and distribute uniformly. One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.