β-globin intergenic transcription and histone acetylation dependent on an enhancer
β-globin intergenic transcription and histone acetylation dependent on an enhancer
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DOI：
10.1128/mcb.02337-06
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发表时间：
2007-04-01
影响因子：
5.3
通讯作者：
Dean, Ann
中科院分区：
文献类型：
--
作者：
Kim, AeRi;Zhao, Hui;Dean, Ann
组蛋白乙酰转移酶与延伸RNA聚合酶II（Pol II）复合物相关，支持组蛋白乙酰化和转录在基因编码序列中机械地交织在一起的观点。在这里，我们研究了组蛋白乙酰化和转录的非编码序列的建立和功能，通过使用一个模型位点连接β-珠蛋白HS 2增强子和胚胎ε-珠蛋白基因在染色质中。募集RNA Pol H的完整HS 2增强子是基因间转录以及增强子和靶基因之间的组蛋白H3乙酰化和K4甲基化所必需的。RNA Pol H募集到靶基因TATA盒不是基因间转录或基因间组蛋白修饰所必需的，强烈暗示它们是增强子赋予的性质。然而，在HS 2处的Pol 11募集、基因间转录和基因间组蛋白修饰不足以转录或修饰靶基因：这些变化需要在基因的TATA盒处起始。结果表明，基因间和基因转录复合体是独立的，可能彼此不同。
Histone acetyltransferases are associated with the elongating RNA polymerase II (Pol II) complex, supporting the idea that histone acetylation and transcription are intertwined mechanistically in gene coding sequences. Here, we studied the establishment and function of histone acetylation and transcription in noncoding sequences by using a model locus linking the beta-globin HS2 enhancer and the embryonic epsilon-globin gene in chromatin. An intact HS2 enhancer that recruits RNA Pol H is required for intergenic transcription and histone H3 acetylation and K4 methylation between the enhancer and target gene. RNA Pol H recruitment to the target gene TATA box is not required for the intergenic transcription or intergenic histone modifications, strongly implying that they are properties conferred by the enhancer. However, Pol 11 recruitment at HS2, intergenic transcription, and intergenic histone modification are not sufficient for transcription or modification of the target gene: these changes require initiation at the TATA box of the gene. The results suggest that intergenic and genic transcription complexes are independent and possibly differ from one another.