ATTRACTION OF TRYPANOSOMA-CRUZI TO VERTEBRATE CELLS INVITRO
ATTRACTION OF TRYPANOSOMA-CRUZI TO VERTEBRATE CELLS INVITRO
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DOI：
10.1111/j.1550-7408.1976.tb03835.x
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
HOWE, CL
中科院分区：
文献类型：
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作者：
DVORAK, JA;HOWE, CL
描述了一种视频技术，其允许量化T的吸引程度。Cruzi锥鞭毛体体外对脊椎动物细胞的作用。牛胚胎骨骼肌细胞（BESM）、HeLa细胞和非洲绿色猴肾Vero细胞都能吸引嗜肌性T.克氏锥鞭毛体。BESM细胞对锥鞭毛体的吸引力比HeLa细胞高2倍，比Vero细胞高10倍。BESM细胞的热失活消除了它们的呼吸能力和吸引T细胞的能力。克氏锥鞭毛体。由于BESM、HeLa和Vero细胞之间的内源性O2消耗没有差异，因此锥虫对3种细胞类型的吸引力差异不太可能是由于细胞周围环境中pO2或pCO2梯度的大小变化所致。
A video technique is described that permits a quantification of the degree of attraction of T. cruzi trypomastigotes to vertebrate cells in vitro. Bovine embryo skeletal muscle cells (BESM), HeLa cells and [African green monkey kidney] Vero cells all attract a myotropic strain of T. cruzi trypomastigotes. BESM cells are 2-fold more attractive to trypomastigotes than HeLa cells and 10-fold more attractive than Vero cells. Heat-inactivation of BESM cells abolishes their ability to respire and also to attract T. cruzi trypomastigotes. As there is no difference in the endogenous O2 consumption between BESM, HeLa and Vero cells, it is unlikely that differences in the attraction of trypomastigotes to the 3 cell types are due to variations in the magnitude of pO2 or pCO2 gradients in the milieu around the cells.