Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage
Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage
复制标题
鉴定传统药用植物对 SDS 诱导的果蝇肠道损伤的保护作用
DOI：
10.3892/etm.2016.3641
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发表时间：
2016-10-01
影响因子：
2.7
通讯作者：
Jin, Li Hua
中科院分区：
文献类型：
--
作者：
Zhou, Yang;Liu, Zonglin;Jin, Li Hua
传统药用植物被广泛用作免疫调节药物，有助于改善健康。总共筛选了50种用于毒性处理的不同植物的体内保护作用。果蝇喂食标准玉米粉-酵母培养基（对照组）或含有药用植物提取物的标准培养基（实验组）。通过用有毒化合物喂养苍蝇来评估存活率。在显微镜下通过绿色荧光蛋白抗体和7-氨基放线菌素D染色分析肠上皮细胞的细胞增殖和死亡。采用定量聚合酶链反应（PCR）检测了不同浓度的有毒化合物对果蝇抗菌肽（AMPs）表达的影响。Nannf（C. pilosula）、风毛菊（Saussurea lappa（Decne.）C. B. Clarke（S. lappa）、白茅Imperata cylindrica Beauv.var.major（Nees）C.E.哈伯（一）圆柱形变种主要）和Melia toosendan Sied。Et Zucc.川楝子（M.toosendan）提高了果蝇存活率，减少了上皮细胞死亡，改善了肠道形态。此外，C.用十二烷基硫酸钠（SDS）处理后，党参提取物诱导抗菌肽（Dpt和Mtk）水平。然而，未观察到这些提取物在体内增加SDS诱导的细胞增殖。这些结果表明，冬虫夏草提取物具有较强的保护作用。pilosula，S.拉帕岛圆柱形变种Major和M.川楝子对果蝇肠细胞的影响。
Traditional medicinal plants are widely used as immunomodulatory medicines that help improve health. A total of 50 different plants used for the treatment of toxicity were screened for their in vivo protective effects. Flies were fed a standard cornmeal-yeast medium (control group) or the standard medium containing medicinal plant extracts (experimental groups). Assessment of the survival rate was performed by feeding flies with toxic compounds. Gut epithelial cells were analyzed for cell proliferation and death by green fluorescent protein antibodies and 7-aminoactinomycin D staining under the microscope. The expression of antimicrobial peptides (AMPs) was evaluated by the quantitative polymerase chain reaction and the results revealed that after feeding the flies with toxic compounds, aqueous extracts from Codonopsis pilosula (Franch.) Nannf (C. pilosula), Saussurea lappa (Decne.) C.B.Clarke (S. lappa), Imperata cylindrica Beauv.var.major (Nees) C.E. Hubb. (I. cylindrical var. major) and Melia toosendan Sied. Et Zucc. (M.toosendan) increased the fly survival rate, reduced epithelial cell death and improved gut morphology. In addition, C. pilosula extracts induced the antimicrobial peptide levels (Dpt and Mtk) following treatment with sodium dodecyl sulfate (SDS). However, these extracts were not observed to increase SDS-induced cell proliferation in vivo. These results indicate that there are strong protective effects in extracts of C. pilosula, S. lappa, I. cylindrical var. major and M. toosendan on Drosophila intestinal cells among 50 medicinal plants.