Increasing the specificity of the forensic luminol test for blood
Increasing the specificity of the forensic luminol test for blood
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DOI：
10.1002/bio.635
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发表时间：
2001-05-01
期刊：
影响因子：
2.9
通讯作者：
Cooper, PD
中科院分区：
文献类型：
--
作者：
Quickenden, TI;Cooper, PD
结果表明，通过测量鲁米诺化学发光的峰值发射波长，可以使假定的鲁米诺化学发光试验具有更强的确定性。当将1g /L含有7g /L过硼酸钠的发光氨水溶液喷洒到含有微量人体血红蛋白的表面时，其发射峰位于455 +/- 2nm处，而将同样的混合物喷洒到含有微量次氯酸钠（家用漂白剂）的表面时，其发射峰位于430 +/- 3nm处。这种光谱差异可以很容易地用光谱设备来确定，这些设备要么在显著的发光衰减发生之前扫描光谱，要么在任何衰减的影响下校正光谱。发现牛血红蛋白和人血红蛋白在光谱上没有显著差异。版权所有John Wiley & Sons， Ltd。
It is shown that the presumptive luminol chemiluminescence test for the presence of traces of blood can be made more determinative by measuring the peak emission wavelength of the luminol chemiluminescence. When sprayed onto a surface containing traces of human haemoglobin, a 1 g/L solution of aqueous luminol containing 7 g/L sodium perborate gives an emission peak at 455 +/- 2 nm, whereas the same mixture gives an emission peak at 430 +/- 3 nm when sprayed onto a surface containing traces of sodium hypochlorite (household bleach). This spectral difference can readily be determined using spectroscopic equipment that either scans the spectrum before significant luminescence decay occurs or corrects the spectrum for the effects of any decay. It was found that bovine haemoglobin and human haemoglobin showed no significant spectral differences. Copyright (C) 2001 John Wiley & Sons, Ltd.