Presence of obestatin in breast milk: Relationship among obestatin, ghrelin, and leptin in lactating women
Presence of obestatin in breast milk: Relationship among obestatin, ghrelin, and leptin in lactating women
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DOI：
10.1016/j.nut.2008.03.020
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发表时间：
2008-07-01
期刊：
影响因子：
4.4
通讯作者：
Sahn, Ibrahim
中科院分区：
文献类型：
--
作者：
Aydin, Suleyman;Ozkan, Yusuf;Sahn, Ibrahim
目的：在血液和母乳中发现了肽激素ghrelin和leptin。本研究旨在探讨母乳中是否也含有肥胖抑制素（与胃饥饿素来自同一基因，但作用相反），并探讨哺乳期母亲血清和乳汁中胃饥饿素、肥胖抑制素和瘦素水平之间的关系。方法：选取健康哺乳期妇女31例，在哺乳前抽取静脉血、初乳和成熟乳。用放射免疫法测定胃饥饿素和肥胖抑制素浓度。采用酶扩增敏感性免疫分析法测定瘦素水平。结果：初乳（538.9 pg/mL）和成熟乳（528.5 pg/mL）中的obstatin水平是相应血液水平（分别为270.3和289.4 pg/mL）的两倍多。相比之下，初乳（2.01 ng/mL）和成熟乳（2.04 ng/mL）中的瘦素水平比相应的血液水平（11.54 ng/mL）低5倍以上。母乳ghrelin水平与瘦素之间无相关性（r = - 0.18, P < 0.05）。而母乳和血液中瘦素水平呈正相关（r = 0.369, P < 0.05）。结论：乳中肥胖抑制素的来源目前尚不清楚，但它来自血液或乳房，并可能通过乳腺进入乳汁。牛奶中的胃饥饿素、肥胖抑制素和瘦素直接影响食欲，其水平可能与调节能量平衡和肥胖的发病机制有关。(C) 2008爱思唯尔公司版权所有。
Objective: The peptide hormones ghrelin and leptin have been found in blood and breast milk. This study was undertaken to investigate whether breast milk also contains obestatin, which is derived from the same gene as ghrelin but has opposite actions, and to characterize the relations among serum and milk ghrelin, obestatin, and leptin levels in lactating mothers.Methods: Venous blood, colostrum, and mature milk were obtained from healthy lactating women (n = 31) just before suckling. The ghrelin and obestatin concentrations were determined by radioimmunoassay. Leptin levels were measured by enzyme-amplified sensitivity immunoassay.Results: Obestatin levels in colostrum (538.9 pg/mL) and mature milk (528.5 pg/mL) were more than twice the corresponding blood levels (270.3 and 289.4 pg/mL, respectively). In contrast, leptin levels in colostrum (2.01 ng/mL) and mature milk (2.04 ng/mL) were more than five-fold lower than the corresponding blood levels (11.54 ng/mL). There was no correlation between breast milk ghrelin levels and leptin (r = - 0.18, P > 0.05). However, there was a positive correlation between leptin levels in breast milk and blood (r = 0.369, P < 0.05).Conclusion: The origin of milk obestatin is not currently known, but it comes from the blood or breast and may drain through the mammary glands into the milk. Ghrelin, obestatin, and leptin in the milk may directly affect appetite and their levels may be related to the regulation of energy balance and the pathogenesis of obesity. (C) 2008 Elsevier Inc. All rights reserved.