Low Power Laser Irradiation Stimulates the Proliferation of Adult Human Retinal Pigment Epithelial Cells in Culture.
Low Power Laser Irradiation Stimulates the Proliferation of Adult Human Retinal Pigment Epithelial Cells in Culture.
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DOI：
10.1007/s12195-008-0041-7
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发表时间：
2009-03
影响因子：
2.8
通讯作者：
Yarmush ML
中科院分区：
文献类型：
--
作者：
Song Q;Uygun B;Banerjee I;Nahmias Y;Zhang Q;Berthiaume F;Latina M;Yarmush ML
我们研究了低功率激光照射对视网膜色素上皮(RPE)细胞增殖的影响。成年人视网膜色素上皮细胞与乌贼黑色素预先孵育，人工着色，暴露于单一亚致死剂量的激光脉冲(590 nm，1微米S，200mJ/cm~2)。随后监测了照射后RPE细胞的DNA合成、细胞数量和生长因子活性。在体外划痕实验中，还研究了亚致死剂量激光照射对RPE单层“伤口”愈合反应的影响。单次脉冲激光照射后6h，色素RPE细胞DNA合成增加。在划痕实验中，激光照射增加了细胞增殖率和创面闭合率。激光照射后48h收集的条件培养液可促进未照射细胞的增殖。辐射可增加视网膜色素上皮细胞分泌血小板衍生生长因子B链，增加多种生长因子及其受体的表达水平，包括血小板衍生生长因子、转化生长因子-β1、碱性成纤维细胞生长因子、表皮生长因子、胰岛素样生长因子以及热休克蛋白。这首次表明，低功率单脉冲激光照射可以刺激RPE细胞的增殖，并上调对RPE细胞具有有丝分裂作用的生长因子。
We investigated the effects of low power laser irradiation on the proliferation of retinal pigment epithelial (RPE) cells. Adult human RPE cells were artificially pigmented by preincubation with sepia melanin, and exposed to a single sublethal laser pulse (590 nm, 1 µs, <200 mJ/cm2). DNA synthesis, cell number, and growth factor activity in irradiated RPE cells were subsequently monitored. The effect of sublethal laser irradiation on the “wound” healing response of an RPE monolayer in an in vitro scratch assay was also investigated. Single pulsed laser irradiation increased DNA synthesis in pigmented RPE cells measured 6 h post-treatment. In the scratch assay, laser irradiation increased the rates of cell proliferation and wound closure. Conditioned medium, collected 48 h following laser treatment, increased cell proliferation of unirradiated cells. Irradiation increased RPE cell secretion of platelet-derived growth factor (PDGF)-B chain, and increased mRNA levels of several growth factors and their receptors, including PDGF, transforming growth factor-β1, basic fibroblast growth factor, epidermal growth factor, insulin-like growth factor, as well as heat shock proteins. This demonstrates, for the first time, that low power single pulsed laser irradiation stimulates the proliferation of RPE cells, and upregulates growth factors that are mitogenic for RPE cells.