Orphanin FQ-induced outward current in rat hippocampus.
Orphanin FQ-induced outward current in rat hippocampus.
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孤啡肽 FQ 在大鼠海马中诱导的外向电流。
DOI：
10.1016/s0006-8993(99)02245-3
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发表时间：
2000
期刊：
影响因子：
2.9
通讯作者：
Takahashi,T
中科院分区：
文献类型：
--
作者：
Amano,T;Matsubayashi,H;Tamura,Y;Takahashi,T
孤啡肽（OFQ）是一种十七肽，在结构上类似于阿片肽。研究表明，孤啡肽受体在海马中有中等密度的结合位点，孤啡肽受体在海马中的表达与学习记忆有关。本研究旨在探讨孤啡肽受体激活是否能诱导培养的大鼠海马神经元超极化。在电流钳模式下，应用OFQ（10−8-10− 5 M）以浓度依赖性方式使培养的海马神经元的膜超极化。此外，在电压钳模式下，应用OFQ（10− 6 M）在海马CA 3锥体神经元中诱导外向电流。在TTX（3×10− 7 M）存在下，在-60 mV的保持电位下，OFQ（10− 6 M）诱导的外向电流偏转的平均最大幅度为24.7±0.54 pA。OFQ诱导的电流在−99.06±3.80 mV（3 mM）时逆转，这非常接近我们标准溶液中K+的能斯特方程（Ek=−96.08 mV，3 mM）计算的K+平衡电位。这表明OFQ诱导的电流是由K+离子介导的。已经证明[Phe 1-（CH 2-NH）Gly 2]Nociceptin（1-13）NH 2）（一种Nociceptin的假肽类似物）和nocistatin是OFQ的选择性拮抗剂。OFQ（10− 6 M）诱导的外向电流可被[Phe 1 <$（CH 2-NH）Gly 2]孤啡肽（1-13）NH 2（10− 5 M）拮抗。与此相反，孤啡肽诱导的外向电流并不被nocistatin（10 - 5 M）所拮抗。这些结果提示海马内存在孤啡肽的生理功能受体。
Orphanin FQ (OFQ) is a heptadecapeptide that structurally resembles opioid peptides. It has been demonstrated that the moderate density of binding sites of OFQ were localized in the hippocampus and that the expression of OFQ receptor in the hippocampus have an important role in learning and memory. This study was designed to investigate whether activation of the OFQ receptor could induced hyperpolarization in the cultured hippocampus neurons in rats. In the current clamp mode, the application of OFQ (10−8–10−5M) hyperpolarized the membranes in cultured hippocampus neurons in a concentration-dependent manner. Moreover, in the voltage clamp mode, application of OFQ (10−6M) induced outward current in hippocampus CA3 pyramidal neurons. In the presence of TTX (3×10−7M), the average maximal amplitude of the outward current deflection induced by OFQ (10−6M) at −60 mV of a holding potential was 24.7±0.54 pA. The OFQ-induced current reversed at −99.06±3.80 mV (3 mM), which was quite close to the K+equilibrium potential as calculated by the Nernst equation (Ek=−96.08 mV, 3 mM) for K+in our standard solution. This suggests that OFQ-induced current was mediated by K+ion. It has been demonstrated that [Phe1ψ(CH2-NH)Gly2]Nociceptin(1–13)NH2) (a pseudopeptide analog of nociceptin), and nocistatin are selective antagonists of OFQ. OFQ (10−6M)-induced outward current was antagonized by application of [Phe1ψ(CH2-NH)Gly2]Nociceptin(1–13)NH2(10−5M). In contrast, OFQ-induced outward current was not antagonized by application of nocistatin (10−5M). These results indicates that there is the physiological functioning receptor of OFQ in the hippocampus.
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噻唑呋林及其硒类似物（2-β-D-呋喃核糖基-4-硒唑甲酰胺）的生化和抗肿瘤活性。
影响因子：
5.8
作者：
T. Boritzki;D. Berry;J. Besserer;P. D. Cook;D. W. Fry;W. Leopold;R. Jackson
通讯作者：
R. Jackson
影响因子：
5.8
作者：
JAYARAM, HN;DION, RL;COONEY, DA
通讯作者：
COONEY, DA
对溶瘤 C-核苷 2-β-D-呋喃核糖基噻唑-4-甲酰胺 (NSC-286193) 的耐药机制。
影响因子：
5.8
作者：
H. Jayaram;D. Cooney;R. Glazer;R. L. Dion;D. Johns
通讯作者：
D. Johns
影响因子：
4.1
作者：
FULLER, SA;HUTTON, JJ;COLEMAN, MS
通讯作者：
COLEMAN, MS
2-β-D-呋喃核糖基噻唑-4-甲酰胺的作用机制研究（NSC 286193）--II。
影响因子：
5.8
作者：
H. Jayaram;A. L. Smith;R. Glazer;D. Johns;D. Cooney
通讯作者：
D. Cooney