Biochemical and histological analysis of "recurrent' macular corneal dystrophy.
Biochemical and histological analysis of "recurrent' macular corneal dystrophy.
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“复发性”黄斑角膜营养不良的生化和组织学分析。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
J. Krachmer
中科院分区：
文献类型：
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作者：
D. Newsome;J. Hassell;M. Rodrigues;A. Rahe;J. Krachmer
一位患有黄斑角膜营养不良的病人，在23年前成功地进行了一次6 mm角膜移植手术，但因明显的不规则散光而接受了第二次角膜移植术。切除的纽扣，其中包含原始移植物和宿主角膜的边缘，被分成几部分。对一部分进行组织学检查，另一部分在器官培养物中与放射性前体和生物合成标记产物一起孵育，第三部分用于细胞培养和染色体核型分析。结果表明，宿主间质细胞没有侵入移植物，移植物间质细胞合成正常的蛋白多糖。此外，尽管宿主间质细胞过度合成异常蛋白多糖，并且该物质在宿主角膜中蓄积，但少量该物质实际上蓄积在移植角膜中，可能导致散光。一个大的移植物，将留下最少的宿主角膜组织可能是导电的黄斑角膜营养不良患者的长期良好的结果。
A patient with macular corneal dystrophy who had a successful 6-mm corneal transplant 23 years ago underwent a second keratoplasty for marked irregular astigmatism. The excised button, which contained the original graft and a rim of host cornea, was divided into several portions. One portion was examined histologically, another portion was incubated in organ culture with radioactive precursors and the biosynthetically labeled products characterized, and a third portion was used for cell culture and karotype analysis. The results indicated that host stromacytes had not invaded the graft and that graft stromacytes had synthesized normal proteoglycans. Furthermore, although there was excessive synthesis of abnormal proteoglycan by host stromacytes and accumulation of this material in the host cornea, minor amounts of this material actually accumulated in the graft cornea, possible contributing to the astigmatism. A large transpant that would leave a minimum of host corneal tissue may be conductive to a longer-term good result in patients with macular corneal dystrophy.