AN ALLOSTERIC HAMMERHEAD RIBOZYME
AN ALLOSTERIC HAMMERHEAD RIBOZYME
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DOI：
10.1038/nbt0295-161
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发表时间：
1995-02-01
期刊：
影响因子：
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通讯作者：
LIZARDI, PM
中科院分区：
文献类型：
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作者：
PORTA, H;LIZARDI, PM
我们构建了一种含有受变构控制的锤头核酶的 RNA 分子。在非活性状态下，RNA 酶无法切割合适的底物。核酶活性状态的形成是由与 DNA 寡核苷酸效应子的特定相互作用触发的，该效应子与 RNA 酶分子中的单链环互补。其他含有不相关核苷酸序列的 DNA 或 RNA 分子不起变构效应器的作用。这项工作证明了设计可响应人工设计的分子识别事件而特异性激活的 RNA 酶的可行性。此类酶可能具有实际应用。
We have constructed an RNA molecule containing a hammerhead ribozyme that is under allosteric control. In the inactive state, the RNA enzyme is unable to cleave a suitable substrate. The formation of the active state of the ribozyme is triggered by a specific interaction with a DNA oligonucleotide effector that is complementary to a single-stranded loop in the RNA enzyme molecule. Other DNA or RNA molecules containing unrelated nucleotide sequences do not function as allosteric effecters. This work demonstrates the feasibility of designing RNA enzymes that are specifically activated in response to an artificially designed molecular recognition event. Such enzymes may have practical applications.