Design and fabrication of multivalent Gal-containing quantum dots and study of its interactions with asialoglycoprotein receptor (ASGP-R)
Design and fabrication of multivalent Gal-containing quantum dots and study of its interactions with asialoglycoprotein receptor (ASGP-R)
复制标题
多价含Gal量子点的设计和制备及其与脱唾液酸糖蛋白受体（ASGP-R）相互作用的研究
DOI：
10.1016/j.tetlet.2010.06.002
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发表时间：
2010-08-11
影响因子：
1.8
通讯作者：
Li, Zhong-Jun
中科院分区：
文献类型：
--
作者：
Yang, Yang;Zhao, Yue-Tao;Li, Zhong-Jun
制备了多价乳糖（Lac-QDs）和半乳糖（Gal-QDs）包覆的CdSeS-ZnS核壳量子点（QDs）。通过核磁共振（NMR）和电感耦合等离子体发射光谱（ICP-OES）确定了糖量子点的分子式。研究了HepG 2细胞对含Gal的糖量子点的摄取。流式细胞术和荧光显微镜分析表明，摄取是受体介导的和选择性的。所制备的多价糖量子点可用于模拟寡糖的肝内吞作用研究。此外，这种类型的糖量子点可以被用作一个有用的荧光探针在细胞成像和分析的碳水化合物-蛋白质相互作用。(C)2010爱思唯尔有限公司保留所有权利。
Multivalent lactose (Lac-QDs)- and galactose (Gal-QDs)- coated CdSeS-ZnS core-shell quantum dots (QDs) were prepared. The formula of the glyco-QDs was determined by nuclear magnetic resonance (NMR) and inductively coupled plasma-optical emission spectrometry (ICP-OES). The uptake of the Gal-containing glyco-QDs by HepG2 cells was investigated. Flow cytometry (FCM) and fluorescence microscopy analysis indicated that the uptake is receptor mediated and selective. The prepared multivalent glyco-QDs could be used to mimic the oligosaccharides in the study of hepatic endocytosis. Furthermore, this type of glyco-QDs can be used as a useful fluorescent probe in cell imaging and analysis of carbohydrate-protein interactions. (C) 2010 Elsevier Ltd. All rights reserved.