Influence of melatonin on basal and gonadotropin-stimulated progesterone and estradiol secretion of cultured human granulosa cells and in the superfused granulosa cell system
Influence of melatonin on basal and gonadotropin-stimulated progesterone and estradiol secretion of cultured human granulosa cells and in the superfused granulosa cell system
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DOI：
10.1159/000052973
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发表时间：
2001-01-01
影响因子：
2.1
通讯作者：
Török, A
中科院分区：
文献类型：
--
作者：
Bódis, J;Koppán, M;Török, A
本研究的目的是探讨褪黑激素 (Me) 对在无血清培养基和灌流 GC 系统中培养的人颗粒细胞 (GC) 的基础和促性腺激素刺激的孕酮 (PG) 和雌二醇 (E-2) 分泌的直接作用。从图宾根大学妇女医院接受体外受精的 34 名女性的排卵前卵泡液中分离出人类 GC。使用放射免疫测定法在存在和不存在Me、普萘洛尔、LH或FSH的情况下测量PG和E-2的产生。通过ANOVA和Newman-Keuls检验对数据进行统计分析。 Me 以剂量依赖性方式刺激 E-2 分泌。普萘洛尔不会引起E-2分泌的任何变化，当与Me一起服用时，它仅部分阻断但不能完全阻止E-2输出。当Me的浓度在10(-4)和10(-8)M之间时，Me对PG产生没有统计学上显着的影响。然而，在10(-3)M时Me显着抑制颗粒细胞的PG输出。 LH 和 FSH 显着刺激两种类固醇激素的分泌。 Me 显着减少 LH 和 FSH 诱导的 E-2 分泌，以及 LH 刺激的 PG 输出，同时仅导致 PG 分泌轻微但显着的减少。在灌注的GC系统中，FSH和LH对PG释放产生显着的刺激作用。 Me 没有改变 FSH 对 PG 的刺激作用，但它导致 LH 刺激的 PG 释放有所延迟。普萘洛尔和 Me 对 PG 释放没有刺激作用。根据我们的结果，我们认为 Me 对人 GC 的基础 E-2 和促性腺激素刺激的 E-2 和 PG 分泌具有直接调节作用。观察到的效应可能在月经周期期间GC功能的调节中发挥生理作用。版权所有 (C) 2001 S. Karger AG，巴塞尔。
The aim of this study was to explore the direct action of melatonin (Me) on basal and gonadotropin-stimulated progesterone (PG) and estradiol (E-2) secretion of human granulosa cells (GCs) cultured in serum-free medium and in a superfused GC system. Human GCs were isolated from preovulatory follicular fluid aspirated from 34 women undergoing in vitro fertilization at the University Women's Hospital of Tubingen. PG and E-2 production was measured in the presence and absence of Me, propranolol, LH or FSH using radioimmunoassay. Statistical analysis of the data was performed by ANOVA and Newman-Keuls test. Me stimulated E-2 secretion in a dose-dependent manner. Propranolol did not cause any change in E-2 secretion, and when given with Me, it only partially blocked but could not entirely prevent E-2 output. There was no statistically significant effect of Me on PG production when Me was administered at concentrations between 10(-4) and 10(-8) M. However, at 10(-3) M Me significantly suppressed PG output of granulosa cells. LH and FSH significantly stimulated the secretion of both steroid hormones. Me significantly reduced LH- and FSH-induced E-2 secretion, as well as LH-stimulated PG output, while it caused only a slight, yet significant decrease in PG secretion. In the superfused GC system, FSH and LH resulted in a significant stimulatory effect on PG release. Me did not modify the stimulatory effect of FSH on PG, while it caused some delay in LH-stimulated PG release. Propranolol and Me had no stimulatory effect on PG release. On the basis of our results we suggest that Me has a direct modulatory effect on basal E-2 and gonadotropin-stimulated E-2 and PG secretion of human GCs. The observed effect may play a physiological role in the regulation of GC function during the menstrual cycle. Copyright (C) 2001 S. Karger AG, Basel.