Changes in intestinal microflora of Caenorhabditis elegans following Bacillus nematocida B16 infection.
Changes in intestinal microflora of Caenorhabditis elegans following Bacillus nematocida B16 infection.
复制标题
杀线虫芽孢杆菌 B16 感染后秀丽隐杆线虫肠道菌群的变化
DOI：
10.1038/srep20178
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发表时间：
2016-02-02
影响因子：
4.6
通讯作者：
Yao L
中科院分区：
文献类型：
--
作者：
Niu Q;Zhang L;Zhang K;Huang X;Hui F;Kan Y;Yao L
病原菌对宿主及其共生微生物群的影响在生物世界中是至关重要和广泛的。土壤中的机会性杀线虫芽孢杆菌B16利用“特洛伊木马”机制杀死秀丽隐杆线虫。B16感染后肠道微生物菌群的变化仍然未知。在这里，我们分析了正常和感染蠕虫的肠道细菌。肠道微生物群落经历了一个复杂的变化后，B16接种，确定通过显着差异的物种多样性，结构，分布和组成之间的未感染和感染蠕虫。不管蠕虫的起源（即，从土壤或腐烂的水果），肠道微生物组的多样性在感染后下降。厚壁菌门的数量急剧增加，而变形菌门、放线菌门、蓝细菌门和酸细菌门的数量均有不同程度的减少。梭菌只存在12小时后感染。感染24 h后，未感染组和感染组分别鉴定出1228和1109种细菌。共有种达21.97%。感染组中芽孢杆菌属的种类较多，而片球菌属、盐单胞菌属、埃希氏菌属和希瓦氏菌属的种类较少（P < 0.01）。因此，本研究首次利用C. elegans作为模式物种。
The effect of pathogenic bacteria on a host and its symbiotic microbiota is vital and widespread in the biotic world. The soil-dwelling opportunistic bacterium Bacillus nematocida B16 uses a “Trojan horse” mechanism to kill Caenorhabditis elegans. The alterations in the intestinal microflora that occur after B16 infection remain unknown. Here, we analyzed the intestinal bacteria presented in normal and infected worms. The gut microbial community experienced a complex change after B16 inoculation, as determined through marked differences in species diversity, structure, distribution and composition between uninfected and infected worms. Regardless of the worm’s origin (i.e., from soil or rotten fruits), the diversity of the intestinal microbiome decreased after infection. Firmicutes increased sharply, whereas Proteobacteria, Actinobacteria, Cyanobacteria and Acidobacteria decreased to different degrees. Fusobacteria was only present 12 h post-infection. After 24 h of infection, 1228 and 1109 bacterial species were identified in the uninfected and infected groups, respectively. The shared species reached 21.97%. The infected group had a greater number of Bacillus species but a smaller number of Pediococcus, Halomonas, Escherichia and Shewanella species (P < 0.01). Therefore, this study provides the first evaluation of the alterations caused by pathogenic bacteria on symbiotic microbiota using C. elegans as the model species.