Numerical analysis of electrophoretic protein patterns of Campylobacter laridis and allied thermophilic campylobacters from the natural environment.
Numerical analysis of electrophoretic protein patterns of Campylobacter laridis and allied thermophilic campylobacters from the natural environment.
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对自然环境中的拉里迪斯弯曲杆菌和相关嗜热弯曲杆菌的电泳蛋白模式进行数值分析。
DOI：
10.1111/j.1365-2672.1988.tb04319.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
F. Bolton
中科院分区：
文献类型：
--
作者：
R. Owen;M. Costas;Lesley Sloss;F. Bolton
对21株菌株进行了细胞蛋白的一维SDS-PAGE分析，其中包括9株弯曲杆菌(9株)、4株对奈立地酸敏感的弯曲菌(NASC)和8株尿素酶阳性的嗜热弯曲菌(UPTC)。UPTC和NASC菌株包括6株来自河水、2株来自贻贝和4株来自海水。供参考的还有其他三种弯曲菌的模式菌株。蛋白质图谱包含45-50条不连续的条带，重复性很高，并被用作两个数值分析的基础。在第一个包含所有蛋白质条带的实验中，21个菌株在80%的相似性(S)水平上聚成了9个类群。典型的拉氏梭菌株被限制在两个物候群(2和5)；非典型菌株分布在其余的物候群中。在第二次分析中，排除了主要蛋白条带(40-48.5kD范围)，21株菌株在80%的S水平上形成了5个聚类群。典型的拉氏隐杆线虫菌株相对同质，属于一个单一的物候群(2)，其中可区分两个亚群。非典型菌株在背景蛋白图谱方面具有更多的异质性，在所有五个表型中都有代表性。在这两种分析的基础上，提出了一种包括六种电泳型的电泳型方案。在第二次分析中，群内S水平较高，并与弯曲杆菌参考菌株分离，表明UPTC和NASC菌株可能属于松材线虫的生物群。
Twenty-one strains comprising Campylobacter laridis (nine), nalidixic acid sensitive campylobacters (NASC) (four), and urease-positive thermophilic campylobacters (UPTC) (eight) were characterized by one-dimensional SDS-PAGE of cellular proteins. The UPTC and NASC strains included six from river water, two from mussels and four from sea water. The type strains of three other Campylobacter species were included for reference. The protein patterns, which contained 45-50 discrete bands, were highly reproducible and were used as the basis for two numerical analyses. In the first, which included all the protein bands, the 21 strains formed nine clusters at the 80% similarity (S) level. The typical C. laridis strains were restricted to two phenons (2 and 5); the atypical strains being distributed among the remaining phenons. In the second analysis, which excluded the principal protein bands (40-48.5 kD range), the 21 strains formed five clusters at the 80% S level. The typical C. laridis strains were relatively homogeneous and fell into a single phenon (2) within which two subgroups were discernable. The atypical strains were more heterogeneous with respect to background protein pattern, with representatives appearing in all five phenons. An electropherotyping scheme comprising six electropherotypes, and based on both analyses is proposed. The high within-group S level and separation from reference strains of Campylobacter in the second analysis, suggested that UPTC and NASC strains belonged within C. laridis possibly as biovars.