Physiological and biochemical changes associated with cotton fiber development - IX. Role of IAA and PAA
Physiological and biochemical changes associated with cotton fiber development - IX. Role of IAA and PAA
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DOI：
10.1016/s0378-4290(02)00062-x
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发表时间：
2002-09-01
影响因子：
5.8
通讯作者：
Thaker, VS
中科院分区：
文献类型：
--
作者：
Gokani, SJ;Thaker, VS
以3个棉花品种Gossypium hirsutum hybrid-4 （H-4）、hybrid-8 （H-8）和G. arboreum G. Cot-15为材料，研究了生长素在体外和体内纤维伸长中的作用。不同品种的终纤维长度存在差异。测定了纤维的生长、游离和共轭吲哚乙酸（IAA）含量和苯基乙酸（PAA）含量。培养抗IAA和PAA抗体，间接ELISA法测定内源性IAA和PAA含量。棉纤维的发育分为四个阶段，即(i)发育期（开花后0-3天），（ii）伸长期（3-24 DPA）， （iii）次生增厚期（18-42 DPA）和（iv）成熟期（42 DPA以后）。在细胞快速伸长阶段，水分含量在纤维进入次生增粗期后先上升后下降。最终纤维长度与游离和共轭IAA和PAA含量呈正相关。在离体生长的纤维中，添加IAA或NAA显著增加了中短纤品种的纤维长度，而PAA仅促进了短纤品种的纤维长度。研究结果表明，生长素在棉纤维发育中起着重要的作用，这可能有助于棉纤维发育基因的选择。(C) 2002 Elsevier Science B.V.版权所有
Fibers of three cotton cultivars Gossypium hirsutum hybrid-4 (H-4), hybrid-8 (H-8), and G. arboreum G. Cot-15 were analyzed to study the role of auxin in fiber elongation, in vitro and in vivo. The cultivars exhibited variation in final fiber length. Fibers were analyzed for growth, free and conjugated indole acetic acid (IAA) content and phenyl acetic acid (PAA) content during the entire period of their development. Antibodies against IAA and PAA were raised and endogenous contents of IAA and PAA were estimated by indirect ELISA. Cotton fiber development is divided into four phases viz. (i) initiation (0-3 days post-anthesis (DPA)), (ii) elongation (3-24 DPA), (iii) secondary thickening (18-42 DPA) and (iv) maturation (42 DPA onwards). During the phase of rapid cell elongation, water content increased and then decreased after the fiber entered the secondary thickening phase, in all three cultivars. A positive correlation between final fiber length and free and conjugated IAA and PAA content was observed. In in vitro grown fiber, media supplemented with IAA or napthyl acetic acid (NAA) significantly increased the length of fiber in middle and short staple cultivars, whereas PAA promoted fiber length in the short staple cultivar only. The results of cultured cotton fiber suggest an important role of auxin in cotton fiber development, which may help for the selection of gene(s) for better fiber development. (C) 2002 Elsevier Science B.V. All rights reserved.