RIBONUCLEOPROTEIN ORGANIZATION OF EUKARYOTIC RNA .19. SMALL NUCLEAR-RNA U2 IS BASE-PAIRED TO HETEROGENEOUS NUCLEAR-RNA
RIBONUCLEOPROTEIN ORGANIZATION OF EUKARYOTIC RNA .19. SMALL NUCLEAR-RNA U2 IS BASE-PAIRED TO HETEROGENEOUS NUCLEAR-RNA
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DOI：
10.1126/science.6178162
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
PEDERSON, T
中科院分区：
文献类型：
--
作者：
CALVET, JP;MEYER, LM;PEDERSON, T
真核细胞含有一组低分子量的核 RNA。其中最丰富的一种被称为 U2 RNA。研究了 U2 RNA 与其他核 RNA 中的互补序列氢键结合的可能性。用补骨脂素衍生物处理培养的人类 (HeLa) 细胞，该衍生物可交联彼此碱基配对的 RNA 链。在变性条件下分离高分子量异质核RNA，并且补骨脂素交联被逆转。对释放的 RNA 进行电泳并与人类克隆的 U2 DNA 探针杂交表明，U2 在体内与异质核 RNA 中的互补序列形成氢键。 U2 RNA 不与核仁 RNA 碱基配对，核仁 RNA 包含核糖体 RNA 的前体。 U2 RNA 可能参与细胞核内 mRNA 的加工。
Eukaryotic cells contain a set of low MW nuclear RNA. One of the more abundant of these is termed U2 RNA. The possibility that U2 RNA is hydrogen-bonded to complementary sequences in other nuclear RNA was investigated. Cultured human (HeLa)cells were treated with a psoralen derivative that cross-links RNA chains that are base-paired with one another. High MW heterogeneous nuclear RNA was isolated under denaturing conditions, and the psoralen cross-links were reversed. Electrophoresis of the released RNA and hybridization with a human cloned U2 DNA probe revealed that U2 is hydrogen-bonded to complementary sequences in heterogeneous nuclear RNA in vivo. U2 RNA is not base-paired with nucleolar RNA, which contains the precursors of ribosomal RNA. U2 RNA may participate in mRNA processing in the nucleus.