The Lethal Dose of Largemouth Bass Virus in Juvenile Largemouth Bass and the Comparative Susceptibility of Striped Bass
The Lethal Dose of Largemouth Bass Virus in Juvenile Largemouth Bass and the Comparative Susceptibility of Striped Bass
复制标题
大口黑鲈病毒对大口黑鲈幼鱼的致死量及条纹鲈的相对敏感性
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
D. Zilberg
中科院分区：
文献类型：
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作者：
J. Plumb;D. Zilberg
摘要大口黑鱼病毒(LMBV)是1994年从美国东南部野生成年大口黑鱼体内分离到的一种虹彩病毒。虽然最初是从濒临死亡的野生鱼类中分离出来的，但它对幼鱼的毒力尚不确定。为了帮助阐明这一点，对幼鱼进行了两次LMBV滴定。肥头鱼细胞中LMBV的滴度分别为104.8和105.8组织培养感染剂量-50%细胞病变终点(TCID_(50))/毫升。在每种细胞培养滴定中使用10倍的LMBV，在连续两次感染性试验中，经腹腔注射(0.1mL/尾)获得计算的致死剂量--50%死亡终点(LD50)分别为282(102.45)和288(102.46)感染量。感染后14d，死鱼和死鱼内脏的平均病毒产量分别为108.5 TCID50/g和107.7 TCID50/g，存活鱼为106.5 TCID50/g。在某种程度上..。
Abstract Largemouth bass virus (LMBV) is an iridovirus that was isolated from wild adult largemouth bass Micropterus salmoides in the southeastern United States in 1994. Although originally isolated from moribund wild fish, its virulence to juvenile largemouth bass is uncertain. To help clarify this point, two LMBV titrations were made in juvenile largemouth bass. Titers of LMBV in fathead minnow cells were 104.8 and 105.8 tissue culture infectious doses—50% cytopathic endpoint (TCID50) per milliliter, respectively. Tenfold serial dilutions of LMBV employed in each cell culture titration, injected intraperitoneally (0.1 mL/fish) into largemouth bass produced calculated lethal dose—50% mortality endpoints (LD50s) of 282 (102.45) and 288 (102.46) infectious doses in two consecutive infectivity trials. Virus yield of assayed infected fish averaged 108.5 TCID50/g and 107.7 TCID50/g in viscera of moribund and dead fish in the two trials and 106.5 TCID50/g in surviving exposed fish 14 d after infection. In a se...