Cyclic tensile stretch load and oxidized low density lipoprotein synergistically induce lectin-like oxidized LDL receptor-1 in cultured bovine chondrocytes, resulting in decreased cell viability and proteoglycan synthesis
Cyclic tensile stretch load and oxidized low density lipoprotein synergistically induce lectin-like oxidized LDL receptor-1 in cultured bovine chondrocytes, resulting in decreased cell viability and proteoglycan synthesis
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DOI：
10.1002/jor.20211
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发表时间：
2006-08-01
影响因子：
2.8
通讯作者：
Hamanishi, Chiaki
中科院分区：
文献类型：
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作者：
Akagi, Masao;Nishimura, Shunji;Hamanishi, Chiaki
已知机械刺激是调节软骨代谢的重要因素。我们测试的假设，凝集素样氧化低密度脂蛋白受体-1（LOX-1）的表达可以调制的软骨细胞的周期性拉伸负荷。在培养的牛关节软骨细胞中，以10个循环/分钟、10 kPa的应力进行6小时的重复拉伸应力的循环加载诱导LOX-1的表达为对照的2.6倍，相当于添加10 μ g/mL氧化低密度脂蛋白（ox-LDL）（对照的2.4倍）。将循环负荷与10 μ g/mL ox-LDL一起沿着施加于软骨细胞导致LOX-1表达协同增加至对照的6.3倍。单独应用循环负荷和10 μ g/mL ox-LDL显著抑制软骨细胞活力（24 h分别为对照组的84.6% ± 3.4%和80.9% ± 3.2%; n = 3; p < 0.05）和蛋白多糖合成[24 h分别为对照组的81.0% ± 7.1%和85.7% ± 5.2%;与天然LDL的94.6%和4.6%相比，p < 0.05（n = 3）]。循环负荷和10 μ g/mL ox-LDL协同影响细胞活力和蛋白多糖合成，在24 h时分别显著抑制至对照组的45.6% ± 4.9%和48.7% ± 6.7%（n 3;与单独应用循环负荷或10 μ g/mL ox-LDL相比，p < 0.01）。在这项研究中，我们证明了循环拉伸负荷和ox-LDL对软骨细胞的细胞活力和蛋白多糖合成的协同作用，这可能是通过增强LOX-1的表达介导的，并在骨关节炎软骨退变的进展中具有重要意义。(c)2006骨科研究学会。出版社：Wiley Periodicals，Inc.
Mechanical stimulation is known to be an essential factor in the regulation of cartilage metabolism. We tested the hypothesis that expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) can be modulated by cyclic tensile stretch load in chondrocytes. Cyclic loading of repeated stretch stress at 10 cycles per minute with 10 kPa of stress for 6 h induced expression of LOX-1 to 2.6 times control in cultured bovine articular chondrocytes, equivalent to the addition of 10 mu g/mL oxidized low density lipoprotein (ox-LDL) (2.4 times control). Application of the cyclic load to the chondrocytes along with 10 mu g/mL ox-LDL resulted in synergistically increased LOX-1 expression to 6.3 times control. Individual application of cyclic loading and 10 mu g/mL ox-LDL significantly suppressed chondrocytes viability (84.6% 3.4% and 80.9% 3.2% of control at 24 h, respectively; n = 3; p < 0.05) and proteoglycan synthesis [81.0% 7.1% and 85.7% 5.2% of control at 24 h, respectively; p < 0.05 when compared with 94.6% 4.6% for native-LDL (n = 3)]. Cyclic loading and 10 mu g/mL ox-LDL synergistically affected cell viability and proteoglycan synthesis, which were significantly suppressed to 45.6% 4.9% and 48.7% 6.7% of control at 24 h, respectively (n 3; p < 0.01 when compared with individual application of cyclic loading or 10 mu g/mL ox-LDL). In this study, we demonstrated synergistic effects of cyclic tensile stretch load and ox-LDL on cell viability and proteoglycan synthesis in chondrocytes, which may be mediated through enhanced expression of LOX-1 and which has important implications in the progression of cartilage degeneration in osteoarthritis. (c) 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.