An electron microscope study of mouse mast cells arising in vivo and in vitro.
An electron microscope study of mouse mast cells arising in vivo and in vitro.
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DOI：
10.1083/jcb.48.3.676
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发表时间：
1971-03
影响因子：
7.8
通讯作者：
Combs, J W
中科院分区：
文献类型：
--
作者：
Combs, J W
Ginsburg和Sachs(5)以及Ginsburg和Lagunoff(4)描述了在光镜水平上与组织肥大细胞形态相同的细胞在由C3H饲养层复盖马血清致敏的C57BI淋巴样细胞组成的小鼠组织培养系统中选择性分化。组织化学评价表明，组织培养来源的肥大细胞颗粒与正常小鼠结缔组织的肥大细胞颗粒一样，含有强酸性粘多糖和一种凝乳胰蛋白酶样酶(4)，进一步表明组织培养来源的肥大细胞与天然肥大细胞具有同源性。在本报告中，肥大细胞之间的同源程度出现在
Selective differentiation of cells morphologically identical with tissue mast cells at the light microscope level has been described by Ginsburg and Sachs (5) and Ginsburg and Lagunoff (4) in a mouse tissue culture system composed of a C3H feeder layer overplated with horse serum-sensitized C57BI lymphoid cells. Histochemical evaluation indicates that the granules of the tissue culturederived mast cells, like the granules of mast cells from normal mouse connective tissue, contain strongly acidic mucopolysaccharides and a chymotrypsin-like enzyme (4), further suggesting a homology between the tissue culture-derived mast cells and native mast cells. In this report, the degree of homology between mast cells arising in