Development of custom-built bone scaffolds using mesenchymal stem cells and apatite-wollastonite glass-ceramics
Development of custom-built bone scaffolds using mesenchymal stem cells and apatite-wollastonite glass-ceramics
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DOI：
10.1089/ten.2007.0124
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发表时间：
2007-12-01
影响因子：
--
通讯作者：
Wood, David J.
中科院分区：
文献类型：
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作者：
Dyson, Jennifer A.;Genever, Paul G.;Wood, David J.
临床上需要新的骨替代材料，其结合了联合收割机长的植入物寿命与完全整合和适当的机械性能。我们已经使用人类间充质干细胞（MSC）填充多孔磷灰石-硅灰石（A-W）玻璃陶瓷支架，该支架通过层制造技术，选择性激光烧结，来制造定制的骨替代品。共聚焦和扫描电子显微镜被用来确定最佳的接种密度，并证明，在21天的培养期内，骨髓间充质干细胞粘附在A-W支架的表面上并保持活力。我们发现在7天内在支架上生长的MSC的数量显著增加。使用溴脱氧尿苷掺入，我们证明了骨髓间充质干细胞在支架上增殖。使用实时PCR，我们分析了成骨标志物碱性磷酸酶，胶原I型，Cbfa-1，骨钙素，骨连接素，骨桥蛋白的表达的骨髓间充质干细胞培养在没有成骨补充剂。骨髓间充质干细胞成骨标志物的表达与A-W支架相当或显著高于组织培养塑料。我们还发现，与商业磷酸钙支架相比，A-W上的碱性磷酸酶活性显著更高。这些结果首次表明A-W支架的生物相容性和骨支持能力及其作为患者特异性骨替代材料的潜力。
There is a clinical need for new bone replacement materials that combine long implant life with complete integration and appropriate mechanical properties. We have used human mesenchymal stem cells (MSCs) to populate porous apatite-wollastonite (A-W) glass-ceramic scaffolds produced by the layer manufacturing technique, selective laser sintering, to create custom-built bone replacements. Confocal and scanning electron microscopy were used to determine optimal seeding densities and to demonstrate that MSCs adhered and retained viability on the surface of A-W scaffolds over a culture period of 21 days. We found a significant increase in the number of MSCs growing on the scaffolds over 7 days. Using bromodeoxyuridine incorporation we demonstrated that MSCs proliferated on the scaffolds. Using real-time PCR we analyzed the expression of the osteogenic markers alkaline phosphatase, collagen type-I, Cbfa-1, osteocalcin, osteonectin, and osteopontin by MSCs cultured in the absence of osteogenic supplements. The expression of the osteogenic markers by MSCs was equivalent to or significantly greater on A-W scaffolds than on tissue culture plastic. We also identified significantly higher alkaline phosphatase activity on A-W compared to a commercial calcium phosphate scaffold. These results indicate for the first time the biocompatibility and osteo-supportive capacity of A-W scaffolds and their potential as patient-specific bone replacement materials.