Cutting edge: Dendritic cells copulsed with microbial and helminth antigens undergo modified maturation, segregate the antigens to distinct intracellular compartments, and concurrently induce microbe-specific Th1 and helminth-specific Th2 responses
Cutting edge: Dendritic cells copulsed with microbial and helminth antigens undergo modified maturation, segregate the antigens to distinct intracellular compartments, and concurrently induce microbe-specific Th1 and helminth-specific Th2 responses
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DOI：
10.4049/jimmunol.172.4.2016
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发表时间：
2004-02-15
影响因子：
4.4
通讯作者：
Pearce, EJ
中科院分区：
文献类型：
--
作者：
Cervi, L;MacDonald, AS;Pearce, EJ
为了检测树突状细胞（DC）区分蠕虫和微生物Ag并诱导适当极化的Th应答的能力，将小鼠DC与蠕虫Ag、虫卵Ag（SF，4）、沿着细菌痤疮丙酸杆菌（Pa）共刺激，并转移到野生型小鼠中。引人注目的是，SEA/Pa-共脉冲DC诱导同时的Pa特异性Th 1（但不是Th 2）应答和SEA特异性Th 2（但不是Th 1）应答。虽然DC暴露于两种Ag经历许多成熟相关的变化，伴随着单独暴露于Pa，Pa诱导的IL-12的产生被SEA抑制。Ag摄取的检查显示，SEA和Pa是通过离散途径获得的，并进入非重叠的细胞内隔室。数据表明，SEA和Pa分离成不同的隔室，加上SEA诱导的DC成熟途径的修改，是DC解释SEA和Pa固有信号的能力的重要组成部分，并诱导适当的极化Th反应。
To examine the ability of dendritic cells (DC) to discriminate between helminth and microbial Ag and induce appropriately polarized Th responses, mouse DC were copulsed with the helminth Ag, schistosome egg Ag (SF,4), along with the bacterium Proprionebacterium acnes, Pa, and transferred into wild-type mice. Strikingly, SEA/Pa-copulsed DC induced concurrent Pa-specific Th1 (but not Th2) responses and SEA-specific Th2 (but not Th1) responses. Although D C exposed to both Ag undergo many of the maturation-associated changes that accompany exposure to Pa alone, Pa-induced IL-12 production was inhibited by SEA. Examination of Ag uptake revealed that SEA and Pa are acquired via discrete pathways and enter non-overlapping intracellular compartments. Data suggest that segregation of SEA and Pa into distinct compartments, coupled with SEA-induced modifications of the DC maturation pathway, are significant components of the ability of DC to interpret signals inherent to SEA and Pa and induce appropriately polarized Th responses.