DETECTION AND LOCALIZATION OF LIPID-PEROXIDATION IN SELENIUM-DEFICIENT AND VITAMIN-E-DEFICIENT RATS USING F-2-ISOPROSTANES
DETECTION AND LOCALIZATION OF LIPID-PEROXIDATION IN SELENIUM-DEFICIENT AND VITAMIN-E-DEFICIENT RATS USING F-2-ISOPROSTANES
复制标题
DOI：
10.1093/jn/124.6.810
复制
发表时间：
1994-06-01
影响因子：
4.2
通讯作者：
BURK, RF
中科院分区：
文献类型：
--
作者：
AWAD, JA;MORROW, JD;BURK, RF
关于维生素E和硒缺乏对体内脂质过氧化作用的影响的数据有限。F-2-异前列腺素是一种新型的前列腺素类，在血浆中游离，在组织中酯化为磷脂，是体内脂质过氧化的标志物。为了解决维生素E和硒在体内防御脂质过氧化的重要性，我们确定了F-2-异前列烷的浓度在血浆和器官的大鼠喂养的饮食缺乏一种或两种营养素。断乳大鼠喂饲维生素E和硒缺乏饲料12周，然后分为四组。其中一组继续给予双缺乏饲料，另三组分别给予维生素E、硒或两者同时添加的饲料（对照饲料），为期4 wk。血浆F-2-异前列腺素在大鼠喂养的双重缺乏的饮食是5.2倍，高于动物改为对照饮食。此外，肝、肺、肾、心脏和骨骼肌磷脂酯化F-2-异前列腺素在这两组之间存在显著差异。在维生素E缺乏饮食的组中观察到较小的增加。硒缺乏单独与更大的脂质过氧化作用无关。脂质过氧化发生在组织中的大鼠喂养的维生素E缺乏的饮食和增加伴随硒缺乏。
Data on the effect of vitamin E and selenium deficiency on lipid peroxidation in vivo have been limited. F-2-isoprostanes are novel prostanoids that, free in plasma and esterified to phospholipids in tissues, are markers of lipid peroxidation in vivo. To address the importance of vitamin E and selenium in defense against lipid peroxidation in vivo, we determined F-2-isoprostane concentrations in the plasma and organs of rats fed diets deficient in one or both nutrients. Weanling rats were fed a vitamin E- and selenium-deficient diet for 12 wk and then divided into four groups. One group continued to receive the doubly deficient diet, and the other three groups were fed the diet supplemented with vitamin E, selenium or both nutrients (control diet) for 4 wk. Plasma F-2-isoprostanes in rats fed the doubly deficient diet were 5.2-fold higher than in animals changed to a control diet. In addition, there were significant differences in liver, lung, kidney, heart and skeletal muscle phospholipid-esterified F-2-isoprostanes between these two groups. Lesser increases were noted in the group fed the vitamin E-deficient diet. Selenium deficiency alone was not associated with greater lipid peroxidation. Lipid peroxidation occurs in tissues of rats fed a vitamin E-deficient diet and is increased by concomitant selenium deficiency.