Population structure delineated with microsatellite markers in fragmented populations of a tropical tree, Carapa guianensis (Meliaceae)
Population structure delineated with microsatellite markers in fragmented populations of a tropical tree, Carapa guianensis (Meliaceae)
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DOI：
10.1046/j.1365-294x.1999.00735.x
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发表时间：
1999-10
影响因子：
4.9
通讯作者：
S. Dayanandan;J. Dole;K. Bawa;R. Kesseli
中科院分区：
文献类型：
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作者：
S. Dayanandan;J. Dole;K. Bawa;R. Kesseli
热带地区的森林砍伐和选择性采伐可能对热带树木种群的遗传过程产生严重后果，影响特定物种以及热带森林群落的长期生存。由于了解人类引起的变化对遗传过程的影响对于制定合理的热带森林群落保护和管理计划至关重要，因此我们为热带树木Carapa guianensis（楝科）开发了微卫星或简单序列重复（SSR）标记，并评估了大型连续森林和选择性砍伐和破碎森林中成年和幼树种群的SSR多态性。多态位点的等位基因数在4 ~ 28个之间。未检测到近亲繁殖的树苗或成人队列，但等位基因丰富度较低的孤立片段的树苗队列。幼树之间的遗传距离、Nei's D和（δµ）2以及遗传距离值均大于成年群体，表明由于森林砍伐和栖息地破碎化，基因流受到限制。这些SSR位点可用于解决许多有关热带树木的种群和保护遗传学的相关问题。
Deforestation and selective logging in the tropics may have serious consequences on genetic processes in tropical tree populations, affecting long‐term survival of a given species as well as tropical forest communities. Because understanding the effects of human‐induced changes on genetic processes is of utmost importance in formulating sound conservation and management plans for tropical forest communities, we developed microsatellite or simple sequence repeat (SSR) markers for the tropical tree Carapa guianensis (Meliaceae) and assessed the polymorphism of SSRs in adult and sapling populations in a large contiguous forest and in selectively logged and fragmented forests. The number of alleles in polymorphic loci ranged between 4 and 28. No inbreeding was detected in saplings or adult cohorts, but the allelic richness was lower in the sapling cohort of the isolated fragment. Genetic distances, Nei’s D and (δµ)2, and RST values among saplings were greater than among adult cohorts, suggesting restriction of gene flow due to deforestation and habitat fragmentation. These SSR loci may be used to address many related questions regarding the population and conservation genetics of tropical trees.