Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene.
Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene.
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细胞周期调节的人类 H4 组蛋白基因上游的蛋白质-DNA 相互作用。
DOI：
10.1126/science.3035717
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Nick,H
中科院分区：
文献类型：
--
作者：
Pauli,U;Chrysogelos,S;Stein,G;Stein,J;Nick,H
细胞周期依赖性组蛋白基因在整个细胞周期的基础水平上转录，在早期S期增加三到五倍。在体内以单核苷酸分辨率分析了细胞周期调节的人类H4组蛋白基因5 '启动子区域的蛋白质- dna相互作用。这个区域包含两个位点，有四个潜在的蛋白质结合区域，在这些区域DNA被保护免受细胞内硫酸二甲酯的反应和细胞核内脱氧核糖核酸酶I的消化。这些蛋白质- dna相互作用在细胞周期的所有阶段持续存在，并与0.16至0.2 mg氯化钠分离。
Cell cycle-dependent histone genes are transcribed at a basal level throughout the cell cycle, with a three- to fivefold increase during early S phase. Protein-DNA interactions in the 5′ promoter region of a cell cycle-regulated human H4 histone gene have been analyzed at single-nucleotide resolution in vivo. This region contains two sites, with four potential protein-binding domains, at which the DNA is protected from reaction with dimethyl sulfate in cells and from digestion with deoxyribonuclease I in nuclei. These protein-DNA interactions persist during all phases of the cell cycle and dissociate with 0.16 to 0.2Msodium chloride.