Comparison of Legionella pneumophila, L. micdadei, L. bozemanii, and L. dumoffii by transmission electron microscopy
Comparison of Legionella pneumophila, L. micdadei, L. bozemanii, and L. dumoffii by transmission electron microscopy
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通过透射电子显微镜比较嗜肺军团菌、米克达德军团菌、博兹曼军团菌和杜莫菲军团菌
DOI：
10.1128/jcm.19.2.116-121.1984
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发表时间：
1984
影响因子：
9.4
通讯作者：
E. Ewing
中科院分区：
文献类型：
--
作者：
G. A. Hébert;C. Callaway;E. Ewing
嗜肺军团菌临床分离株。米克达代湖bozemanii和L. dumoffii在活性培养基接种物的炭-酵母提取物琼脂上生长，并通过三种不同的方法制备用于透射电子显微镜检查。所有四种军团菌的细胞具有细胞质空泡，革兰氏阴性类型的细胞包膜与致密的肽聚糖样层，钌红阳性多糖胶囊，和一个单一的亚极鞭毛。在L. micdadei与外膜之间被一层电子透明物质隔开，而这层物质在其他被检测物种的细胞中是不存在的。
Clinical isolates of Legionella pneumophila, L. micdadei, L. bozemanii, and L. dumoffii were grown on charcoal-yeast extract agar from a living-medium inoculum and prepared for transmission electron microscopy by three different methods. Cells of all four Legionella species possessed cytoplasmic vacuoles, a gram-negative type of cell envelope with a dense peptidoglycan-like layer, a ruthenium red-positive polysaccharide capsule, and a single subpolar flagellum. The dense polysaccharide capsule seen on cells of L. micdadei was separated from the outer membrane by an extra layer of electron-lucent material that was not present on cells of the other species examined.