Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages.
Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages.
复制标题
大鼠细胞因子合成抑制因子 (IL-10) cDNA 的分子克隆及其在脾脏和巨噬细胞中的表达。
DOI：
10.1006/bbrc.1993.1436
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发表时间：
1993
影响因子：
3.1
通讯作者：
Wilson,CB
中科院分区：
文献类型：
--
作者：
Feng,L;Tang,WW;Chang,JC;Wilson,CB
克隆了大鼠细胞因子合成抑制因子（IL-10）。与小鼠和人IL-10（mIL-10，hIL-10）一样，大鼠IL-10与EB病毒（BCRFI）中的开放阅读框显示出很强的DNA和氨基酸序列同源性。转染大鼠IL-10的COS-7细胞上清可抑制小鼠Th 1细胞产生IFN-γ。在RNA酶保护测定中使用来自大鼠IL-10的探针，以证明IL-10在LPS刺激的大鼠脾和FACS纯化的OX-42反应性腹膜巨噬细胞中表达。如本研究所示，巨噬细胞产生IL-10，表明IL-10可能在炎症中具有自分泌功能。
Rat cytokine synthesis inhibitory factor (IL-10) was cloned. Like mouse and human IL-10 (mIL-10, hIL-10), rat IL-10 exhibits strong DNA and amino acid sequence homology to the open reading frame in the Epstein-Barr virus, BCRFI. The supernatant of COS-7 transfectants with rat IL-10 was found to inhibit the production of IFN-γ by murine Th1 cells. A probe from the rat IL-10 was used in an RNase protection assay to demonstrate that IL-10 was expressed in LPS-stimulated rat spleen and FACS-purified OX-42-reactive peritoneal macrophages. The production of IL-10 by macrophages, as shown in the current study, suggests that IL-10 may have an autocrine function in inflammation.