Lectin-Based Nanoprobes Functionalized with Enzyme for Highly Sensitive Electrochemical Monitoring of Dynamic Carbohydrate Expression on Living Cells
Lectin-Based Nanoprobes Functionalized with Enzyme for Highly Sensitive Electrochemical Monitoring of Dynamic Carbohydrate Expression on Living Cells
复制标题
用酶功能化的基于凝集素的纳米探针，用于高灵敏电化学监测活细胞动态碳水化合物表达
DOI：
10.1021/ac902285q
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发表时间：
2010-02-15
影响因子：
7.4
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
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作者：
Ding, Lin;Ji, Quanjiang;Ju, Huangxian
设计了一种双功能化纳米探针，通过整合金纳米粒子对碳水化合物的特异性识别和蛋白质的酶促信号扩增，实现了对活细胞碳水化合物的高灵敏度和选择性原位评价。在电极表面制备了精氨酸-甘氨酸-天冬氨酸-丝氨酸四肽功能化的纳米角纳米支架，用于细胞捕获和增强电连通性。结合纳米探针和肽纳米角，开发了一种高灵敏度的细胞传感电化学策略，该策略具有检测限低至15个细胞、动态范围宽、可接受的速度和低成本等优点。该方法进一步用于监测肿瘤细胞对药物反应时碳水化合物表达的动态变化，避免了细胞的破坏或标记以及凝集素和酶的共价标记。三组分的一锅偶联非常方便，可推广用于制备其他凝集素功能化纳米探针。这一技术的进一步发展将大大有助于应对全面理解糖糖密码的挑战。
A dual-functionalized nanoprobe was designed for highly sensitive and selective in situ evaluation of carbohydrates on living cells by integrating the specific carbohydrate recognition and enzymatic signal amplification of proteins on Au nanoparticles. A nanoscaffold of nanohorns functionalized with arginine-glycine-aspartic acid-serine tetrapeptide was also prepared on an electrode surface for cell capture and enhancing the electrical connectivity. Combined with the nanoprobe and peptide-nanohorns, a highly sensitive electrochemical strategy was developed for cytosensing, which showed a detection limit down to 15 cells, broad dynamic range, acceptable rapidity, and low cost. The proposed method was further used for monitoring of dynamic variation of carbohydrate expression on cancer cells in response to drugs, which obviated the destruction or labeling of cells and the covalent tagging of lectin and enzyme. The one-pot conjugation of three components was very convenient and could be extended for preparation of other lectin-functionalized nanoprobes. Further development of this technique would contribute considerably to meeting the challenges in comprehensive understanding of the glycomic codes.