Detection and quantification of cytokines and other biomarkers.
Detection and quantification of cytokines and other biomarkers.
复制标题
DOI：
10.1007/978-1-61779-527-5_2
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Remick D
中科院分区：
文献类型：
--
作者：
Chiswick EL;Duffy E;Japp B;Remick D
细胞因子浓度的精确测量是研究炎症的重要手段。酶联免疫吸附试验（ELISA）是一种简单、低成本的分析工具，可提供体外或体内细胞因子研究所需的特异性和敏感性。本文描述了一种系统的方法来开发间接夹心ELISA，以准确和精确地检测和量化细胞因子或其他生物标志物。还详细说明了使用顺序ELISA法分析有限体积样品中的多种细胞因子。最后，考虑到成本和开发所需的额外时间，讨论了多重ELISA的概念。
Accurate measurement of cytokine concentrations is a powerful and essential approach to the study of inflammation. The enzyme-linked immunosorbent assay (ELISA) is a simple, low-cost analytical tool that provides both the specificity and sensitivity required for the study of cytokines in vitro or in vivo. This communication describes a systematic approach to develop an indirect sandwich ELISA to detect and quantify cytokines, or other biomarkers, with accuracy and precision. Also detailed is the use of sequential ELISA assays to analyze multiple cytokines from samples with limited volumes. Finally, the concept of a multiplex ELISA is discussed with considerations given to cost and additional time required for development.