Effects of UV-B on Secondary Metabolites of St. John's Wort (Hypericum perforatum L.) Grown in Controlled Environments
Effects of UV-B on Secondary Metabolites of St. John's Wort (Hypericum perforatum L.) Grown in Controlled Environments
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DOI：
10.1111/j.1751-1097.2011.00904.x
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发表时间：
2011-05-01
影响因子：
3.3
通讯作者：
Weston, Leslie A.
中科院分区：
文献类型：
--
作者：
Brechner, Melissa L.;Albright, Louis D.;Weston, Leslie A.
由于活性成分（AI）浓度与标签上声称的值存在很大差异，药用植物行业正受到越来越多的审查。这种差异的原因包括影响AI浓度的环境和基因型变异。圣约翰草（贯叶连翘）是一种流行的草药，其在产品之间也表现出AI浓度的显著差异。本研究评价了三种生物活性代谢产物的浓度变化。在暴露于UV光后，植物仍然是营养的。用55天龄的植株在400 μ mol m（-2）s（-1）PAR下每天生长16小时进行处理。评价了三种UV光处理：单次剂量、每日剂量和每日剂量递增。在每次处理后监测金丝桃素、假金丝桃素和金丝桃素的浓度7天。与单次给药治疗相比，每日剂量和每日剂量增加未导致次级代谢物显著增加。这些结果表明，在H.可以通过UV光暴露诱导穿孔。从这项研究中获得的信息可以用于优化受控环境中的总生物量和代谢产物的生产。
The medicinal plant industry is under increasing scrutiny due to wide variance in active ingredient (AI) concentration from values claimed on labels. Reasons for this disparity include environmental and genotypic variation which influence AI concentration. St. John's wort (Hypericum perforatum) is a popular herbal remedy which also exhibits marked variance in AI concentration among products. This study evaluated concentration changes of three biologically active metabolites of H. perforatum after exposure to UV light while plants were still vegetative. Treatments were performed with 55-day-old plants grown under 400 mu mol m(-2) s(-1) PAR for 16 h a day. Three UV light treatments were evaluated: a single dose, a daily dose and an increasing daily dose. Concentrations of hyperforin, pseudohypericin and hypericin were monitored for 7 days after each treatment. A daily dose and an increasing daily dose did not produce significantly greater increases in secondary metabolites compared to single dose treatments. These results suggest the small but significant transient metabolite concentration increases in H. perforatum can be induced by UV light exposure. Information from this study can be useful in optimizing total biomass and metabolite production in controlled environments.