REO1 and ROX1 are alleles of the same gene which encodes a transcriptional repressor of hypoxic genes in Saccharomyces cerevisiae.
REO1 and ROX1 are alleles of the same gene which encodes a transcriptional repressor of hypoxic genes in Saccharomyces cerevisiae.
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REO1 和 ROX1 是同一基因的等位基因，编码酿酒酵母中缺氧基因的转录抑制因子。
DOI：
10.1007/s002940050291
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发表时间：
1997
期刊：
影响因子：
2.5
通讯作者：
Poyton,RO
中科院分区：
文献类型：
--
作者：
Kwast,KE;Burke,PV;Brown,K;Poyton,RO
REO 1和ROX 1基因被认为是酿酒酵母中血红素依赖的低氧基因转录抑制因子，然而，遗传互补研究得出了关于这两个基因是相同还是不同的相互矛盾的结果。由于这些阻遏物的核心重要性，控制几乎所有已知的缺氧基因在酵母中的表达，我们试图解决这个混乱的reo 1 androx 1突变体的表型比较使用Northern印迹分析，进行额外的互补研究，并测序theROX 1基因inreo 1菌株。Northern-blot分析的areo 1菌株显示野生型表达的需氧基因检查，但Rox 1调节，缺氧基因的去阻遏。这些低氧基因的有氧转录水平也去抑制在一个二倍体菌株通过交配rox 1破坏菌株与areo 1菌株，表明这两个菌株之间没有发生遗传互补。reo 1株ROX 1基因序列分析表明，其编码区5′端发生移码突变，第6位为无义密码子。总之，这些结果提供了令人信服的证据，reo 1是ROX 1的等位基因。
Both theREO1andROX1genes are thought to encode heme-dependent, transcriptional repressors of hypoxic genes inSaccharomyces cerevisiae.However, genetic complementation studies have yielded conflicting results about whether these are the same or different genes. Because of the central importance of these repressors, which control the expression of nearly all known hypoxic genes in yeast, we have sought to resolve this confusion by comparing the phenotypes ofreo1androx1mutants using Northern-blot analyses, performing additional complementation studies, and sequencing theROX1gene inreo1strains. Northern-blot analyses of areo1strain show wild-type expression of the aerobic genes examined, but de-repression of the Rox1-regulated, hypoxic genes. Aerobic transcript levels of these hypoxic genes were also de-repressed in a diploid strain created by mating a rox1disrupted strain with areo1strain, indicating that genetic complementation did not occur between these two strains. Sequence analyses ofROX1inreo1strains reveal a frame-shift mutation in the 5′-end of its coding region, resulting in a nonsense codon in the sixth position. Taken together, these results provide compelling evidence that reo1is an allele ofROX1.