Effectiveness of ultrasonic cleaning of dental instruments.
Effectiveness of ultrasonic cleaning of dental instruments.
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DOI：
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发表时间：
1995-06
影响因子：
1.4
通讯作者：
W. Cafruny;A. Brunick;D. Nelson;R. Nelson
中科院分区：
文献类型：
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作者：
W. Cafruny;A. Brunick;D. Nelson;R. Nelson
目的定量检测常规预防用牙科器械的血液污染情况，评价超声去污对减少口腔器械血液污染和病毒污染的效果。材料与方法采用免疫球蛋白作为血液标记物，对常规预防后的口腔器械进行血液污染分析。结果估计的污染血量一般在1.4×10(-6)至2.0×10(-4)ml之间，将仪器与血液污染饱和的尝试表明，最大可能的保留血量约为正常污染水平的10倍。用手擦洗受污染的仪器在清除血液污染方面相对无效且不一致。超声波清洁器的去污比洗手更有效，从而将血液污染减少了100倍以上。使用小鼠模型病毒(乳酸脱氢酶提升病毒，LDV)，通过残留病毒检测，获得了牙科器械和牙科手机的高水平病毒污染。超声波治疗将牙科器械上的病毒污染水平降低了一百万倍，牙科手机中的病毒污染水平降低了一千倍。这些结果提供了对人类暴露于牙科器械的感染威胁及其通过超声减少感染威胁的定量估计。
PURPOSE To quantitate blood contamination present on dental instruments used for routine prophylaxis and to assess the effectiveness of ultrasonic decontamination in reducing blood and virus contamination on dental instruments. MATERIALS AND METHODS Human blood contamination present on dental instruments obtained after routine prophylaxis was analyzed using IgG as a blood marker. RESULTS The estimated contaminating blood volume was found to normally range between 1.4 x 10(-6) to 2.0 x 10(-4) ml. Attempts to saturate the instruments with blood contamination suggested that the maximum possible retained blood volume was about 10-fold higher than the normal levels of contamination. Hand scrubbing of contaminated instruments was both relatively ineffective and inconsistent in removing blood contamination. Decontamination in an ultrasonic cleaner was more effective than hand washing, resulting in greater than a 100-fold reduction of blood contamination. Using a mouse model virus (lactate dehydrogenase-elevating virus, LDV), high levels of virus contamination of dental instruments and dental handpieces were achieved, as determined by assay of residual virus. Ultrasonic treatment reduced the level of virus contamination present on dental instruments by one million-fold, and virus contamination present in dental handpieces was reduced by one thousand-fold. These results provide quantitative estimations of the infection threat and its reduction by ultrasonication, posed by human-exposed dental instruments.