The effect of non-specific interactions on cellular adhesion using model surfaces
The effect of non-specific interactions on cellular adhesion using model surfaces
复制标题
DOI：
10.1016/j.biomaterials.2004.05.026
复制
发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Ducheyne, P
中科院分区：
文献类型：
--
作者：
Lee, MH;Brass, DA;Ducheyne, P
使用旋转圆盘装置测量细胞和模型功能表面之间的非特异性相互作用的贡献。这些模型的功能表面创建使用自组装单层（SAM）的烷基硅烷封端的环氧基，羧基（COOH），胺（NH 2，），和甲基（CH 3）基团。这些自组装膜的特征在于使用椭圆偏振。原子力显微镜、接触角测角法和X射线光电子能谱法，以确认存在具有预期理化特性的良好形成的单层。所有基材在长时间暴露于水性条件下（长达18小时）也表现出优异的稳定性。K100红白血病细胞对功能性基质的粘附强度有如下趋势：CH 3 < COOH接近环氧化物，远小于NH 2。NH2 SAM表面表现出几乎一个数量级更大的粘附强度比其他SAM和这种非特异性的影响超过了测量的粘附时，也固定在表面上的RGD三肽。这些发现说明了底物选择的重要性，在肽介导的细胞粘附的定量研究。(C)2004爱思唯尔有限公司保留所有权利。
The contribution of non-specific interactions between cells and model functional surfaces was measured using a spinning disc apparatus. These model functional surfaces were created using self-assembled monolayers (SAM) of alkylsilaness terminated with epoxide, carboxyl (COOH), amine (NH2,), and methyl (CH3) groups. These SAMs were characterized using ellipsometry. atomic force microscopy, contact angle goniometry, and X-ray photoelectron spectroscopy to confirm the presence of well-formed monolayers of expected physicochemical characteristics. All substrates also demonstrated excellent stabitity under prolonged exposure (up to 18h) to aqueous conditions. The adhesion strength of K100 erythroleukemia cells to the functional substrates followed the trend: CH3 < COOH approximate to epoxide much less than NH2. The NH2 SAM surface exhibited nearly an order of magnitude greater adhesion strength than the other SAMs and this non-specific effect exceeded the adhesion measured when RGD tri-peptides were also immobilized on the surface. These findings illustrate the importance of substrate selection in quantitative studies of peptide-mediated cellular adhesion. (C) 2004 Elsevier Ltd. All rights reserved.