High MR sensitive fluorescent magnetite nanocluster for stem cell tracking in ischemic mouse brain
High MR sensitive fluorescent magnetite nanocluster for stem cell tracking in ischemic mouse brain
复制标题
高磁共振敏感荧光磁铁矿纳米簇用于缺血小鼠大脑干细胞追踪
DOI：
10.1016/j.nano.2011.03.006
复制
发表时间：
2011-12-01
影响因子：
5.4
通讯作者：
Yang, Guo-Yuan
中科院分区：
文献类型：
--
作者：
Wang, Yongting;Xu, Fenghua;Yang, Guo-Yuan
干细胞在治疗疾病和损伤，包括缺血性脑损伤方面显示出巨大的潜力。然而，开发用于长期跟踪干细胞的药物而副作用很少仍然具有挑战性。我们的目的是开发一种新的荧光磁铁矿纳米簇（FMNC）具有高MRI灵敏度，并检查其在标记和跟踪间充质干细胞（MSC）的应用。为此，我们开发了FMNC，通过将单个磁铁矿纳米颗粒（NPs）嵌入涂有两层二氧化硅和一层罗丹明夹层的聚苯乙烯支架中。我们研究了FMNC在MSC标记中的功效以及在缺血小鼠脑中追踪FMNC标记的MSC的可行性。我们发现FMNC具有高的细胞标记效率，对MSC没有不良影响。在小鼠大脑中动脉闭塞模型中，经FMNC标记的MSC移植后，FMNC标记的MSC迁移并聚集在缺血区。MRI结果与免疫组化结果高度相关。来自临床编辑：在这项研究中，作者报告了一种新型的荧光磁铁矿纳米簇，具有高MRI灵敏度和标记和跟踪间充质干细胞，并提供了利用小鼠中风模型的体内数据。(C)2011 Elsevier Inc. All rights reserved.
Stem cells have shown a great potential to treat diseases and injuries, including ischemic brain injury. However, developing agents for the long-term tracking of stem cells with few side effects is still challenging. Our aim is to develop a novel fluorescent-magnetite-nanocluster (FMNC) with high MRI sensitivity and to examine its application in the labeling and tracking of mesenchymal stem cells (MSC). For this purpose, we developed FMNC by embedding individual magnetite nanoparticles (NPs) into a polystyrene scaffold coated with two layers of silica and a sandwiched layer of rhodamine. We examined the efficacy of FMNC in MSC labeling and the feasibility of tracking FMNC-labeled MSCs in the ischemic mouse brain. We found that FMNC has high cell-labeling efficiency with no adverse effects on MSCs. In a mouse middle cerebral artery occlusion model, FMNC-labeled MSCs migrated to and accumulated in the ischemic region after FMNC-labeled MSC transplantation. MRI findings highly correlated to immunohistochemistry results.From the Clinical Editor: In this study, the authors report a novel fluorescent-magnetite-nanocluster with high MRI sensitivity and to labeling and tracking of mesenchymal stem cells, and provide in vivo data utilizing a murine stroke model. (C) 2011 Elsevier Inc. All rights reserved.