Relevance of density, size and DNA content of tumour cells to the lung colony assay.
Relevance of density, size and DNA content of tumour cells to the lung colony assay.
复制标题
肿瘤细胞的密度、大小和 DNA 含量与肺集落测定的相关性。
DOI：
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发表时间：
1977
影响因子：
8.8
通讯作者：
M L Meistrich
中科院分区：
文献类型：
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作者：
D. Grdina;W. Hittelman;R A White;M L Meistrich
用rengrafin的线性密度梯度离心分离小鼠纤维肉瘤，并将其分离成活细胞亚群。在肺集落试验中，这两个亚群被称为2带和4带，它们的克隆生成能力不同。密度较低的Band 2细胞比Band 4细胞的克隆性更强（分别为2.9%和1.4%）。每个条带根据细胞的大小通过离心洗脱进一步分离。含能带2的各大小类细胞的克隆生成能力均高于含能带4的相应大小类细胞。因此，细胞大小的差异不是造成这些条带间克隆性差异的原因。为了确定肿瘤细胞的细胞周期分布是否与克隆效率的差异有关，采用了流动微荧光法和过早染色体凝聚法。未分离组和带4组肿瘤细胞在S和G2中的比例高于带2组，但许多S和G2肿瘤细胞表现出广泛的染色体损伤。从这项研究中，我们得出结论，较轻的肿瘤细胞的克隆生成能力的增加不是由于细胞大小或细胞周期参数的差异。
Mouse fibrosarcoma tumours were dissociated and divided into subpopulations of viable cells by centrifugation in linear density gradients of Renografin. Two of these subpopulations, designated Band 2 and Band 4, differed in their clonogenic ability in lung colony assay. The less dense Band 2 cells were significantly more clonogenic than the Band 4 cells (2.9 percent vs 1.4 percent respectively). Each band was further separated on the basis of cell size by centrifugal elutriation. Each size class of cells comprising Band 2 showed higher clonogenic ability than the corresponding size class in Band 4. Thus cell size differences were not responsible for the clonogenic differences between these bands. To determine whether cell-cycle distribution of the tumour cells was responsible for differences in cloning efficiency, flow microfluorometric and premature chromosome condensation methods were utilized. The unseparated and Band 4 populations showed a higher percentage of cells in S and G2 than did the Band 2 populations, but many of the S and G2 tumour cells showed extensive chromosome damage. From this study we conclude that the increased clonogenic ability of the lighter tumour cells is not due to differences in cell size or cell-cycle parameters.