Effects of developmental exposure to bisphenol A on spatial navigational learning and memory in rats: A CLARITY-BPA study.
Effects of developmental exposure to bisphenol A on spatial navigational learning and memory in rats: A CLARITY-BPA study.
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DOI：
10.1016/j.yhbeh.2015.09.005
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发表时间：
2016-04
影响因子：
3.5
通讯作者：
Rosenfeld CS
中科院分区：
文献类型：
--
作者：
Johnson SA;Javurek AB;Painter MS;Ellersieck MR;Welsh TH Jr;Camacho L;Lewis SM;Vanlandingham MM;Ferguson SA;Rosenfeld CS
双酚A（BPA）是一种普遍存在的工业化学品，用于生产各种物品。先前的研究表明BPA暴露可能导致神经破坏性影响;然而，动物和人类研究的数据不一致。作为BPA毒性学术和监管见解联盟（Consortium Linking Academic and Regulatory Insights on BPA Toxicity，简称BPA）的一部分，我们试图确定发育中暴露于BPA的雌性和雄性大鼠是否表现出后来的空间导航学习和记忆缺陷。妊娠NCTR Sprague-Dawley大鼠从妊娠第6天至分娩期间经口给药，后代直接经口给药直至断奶（出生后第21天）。治疗组包括溶剂对照、三种BPA剂量（2.5 μg/kg/天-[2.5]、25 μg/kg/天-[25]和2500 μg/kg/天-[2500]）和0.5 μg/kg/天炔雌醇（EE）-参考雌激素剂量。在成年期，在巴恩斯迷宫中对1只/性别/窝进行7天测试。2500 BPA组在第7天比对照组、2.5 BPA组和EE组嗅到更多的错误孔。2500 BPA雌性动物在规定时间内找到逃生盒的可能性低于对照组雌性动物（P值= 0.04）。尽管2.5 BPA雌性动物表现出延长的潜伏期，但效果未达到显著性（P值= 0.06），而2.5 BPA雄性动物与对照雄性动物相比表现出改善的潜伏期（P值= 0.04），尽管该结果的显著性尚不确定。在任何雄性或雌性给药组中均未检测到血清睾酮浓度差异。目前的研究结果表明，大鼠发育暴露于BPA可能会破坏空间导航学习和记忆的各个方面。
Bisphenol A (BPA) is a ubiquitous industrial chemical used in the production of a wide variety of items. Previous studies suggest BPA exposure may result in neuro-disruptive effects; however, data are inconsistent across animal and human studies. As part of the Consortium Linking Academic and Regulatory Insights on BPA Toxicity (CLARITY-BPA), we sought to determine whether female and male rats developmentally exposed to BPA demonstrated later spatial navigational learning and memory deficits. Pregnant NCTR Sprague-Dawley rats were orally dosed from gestational day 6 to parturition, and offspring were directly orally dosed until weaning (postnatal day 21). Treatment groups included a vehicle control, three BPA doses (2.5 μg/kg/day-[2.5], 25 μg/kg/day-[25], and 2500 μg/kg/day-[2500]) and a 0.5 μg/kg/day ethinyl estradiol (EE)-reference estrogen dose. At adulthood, 1/sex/litter was tested for seven days in the Barnes maze. The 2500 BPA group sniffed more incorrect holes on day 7 than those in the control, 2.5 BPA, and EE groups. The 2500 BPA females were less likely than control females to locate the escape box in the allotted time (P value= 0.04). Although 2.5 BPA females exhibited a prolonged latency, the effect did not reach significance (P value = 0.06), whereas 2.5 BPA males showed improved latency compared to control males (P value = 0.04), although the significance of this result is uncertain. No differences in serum testosterone concentration were detected in any male or female treatment groups. Current findings suggest developmental exposure of rats to BPA may disrupt aspects of spatial navigational learning and memory.