Leydig insulin-like hormone, gubebnacular development and testicular descent
Leydig insulin-like hormone, gubebnacular development and testicular descent
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DOI：
10.1016/s0022-5347(05)66389-6
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发表时间：
2001-05-01
影响因子：
6.6
通讯作者：
Hutson, JM
中科院分区：
文献类型：
--
作者：
Kubota, Y;Nef, S;Hutson, JM
目的：睾丸下降由2个形态和激素步骤控制。经腹睾丸下降由引带肿胀和颅悬韧带退化介导。苗勒管抑制物质（MIS）已被提议刺激肿胀，但这仍然存在争议。最近，一种Leydig胰岛素样激素（Insl 3）突变小鼠被发现具有隐睾和引带缺陷。材料和方法：在胚胎第16.5天和出生时检测Insl 3纯合子（-/-）、杂合子（+/-）和野生型（+/+）小鼠的睾丸位置和引带的发育。结果：11只Ins 13纯合子雄性小鼠在胚胎第16.5天和出生时睾丸下降明显受损（P
Purpose: Testicular descent is controlled by 2 morphological and hormonal steps. Transabdominal testicular descent is mediated by gubernacular swelling and regression of the cranial suspensory ligament. Mullerian inhibiting substance (MIS) has been proposed to stimulate the swelling but this remains controversial. Recently, a mouse mutant for Leydig insulin-like hormone (Insl3) was found to have undescended testis and deficient gubernaculum. We examine the testicular position of Insl3 mutant mice and the development of gubernacula.Materials and Methods: Mice with Insl3 homozygotes (-/-), heterozygotes (+/-) and wild-types (+/+) were examined at embryonic day 16.5 and birth. Macroscopic dissections and measurements of the testicular position, as well as microscopic analysis (hematoxylin and eosin, and Masson's trichrome) were performed.Results: Of the mice 11 Ins13 homozygote males had significantly impaired testicular descent at embryonic day 16.5 and birth (p