Effect of pressure on cultured smooth muscle cells.
Effect of pressure on cultured smooth muscle cells.
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DOI：
10.1016/s0024-3205(97)00603-6
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发表时间：
1997-08
期刊：
影响因子：
6.1
通讯作者：
M. Watase;M. Awolesi;J. Ricotta;B. Sumpio
中科院分区：
文献类型：
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作者：
M. Watase;M. Awolesi;J. Ricotta;B. Sumpio
最近的研究表明，平滑肌细胞（SMC）的生长和形态可以通过重复应变调制。然而，很少有人知道压力的影响，没有相关的细胞拉伸，对SMC表型。为了研究这一点，培养的牛主动脉SMC生长在刚性表面上，并放置在一个定制设计的有机玻璃压力室，仔细调节5%CO2/空气环境。SMC暴露于大气，105毫米汞柱或120 - 90毫米汞柱的压力，频率为60个周期/分钟（0.5秒收缩，0.5秒收缩）。在第1、3、5、7和9天测定SMC数量。暴露于压力的SMC更加细长，并且到第5天细胞数量显着增加，并持续到第9天。条件培养基中的乳酸脱氢酶（LDH）（细胞毒性指标）在每个时间点组间无差异。各组SMC培养液的pH值和pCO 2值也无差异。这是第一次报告的影响，增加静态和脉动压力对SMC在体外，并表明增加增殖率。我们推测，血管在体内暴露的全身压力可能对平滑肌细胞的表型有显著的调节作用，这可能会影响SMC对损伤或刺激的反应。
Recent studies indicate that smooth muscle cell (SMC) growth and morphology can be modulated by repetitive strain. However, there is very little known about the influence of pressure, without an associated cell stretch, on SMC phenotype. To study this, cultured bovine aortic SMC were grown on a rigid surface and placed in a custom-designed plexiglass pressure chamber with a carefully regulated 5% CO2/air environment. SMC were exposed to either atmospheric, 105 mm Hg or 120 90 mm Hg pressure at a frequency of 60 cycles/min (0.5 s systole, 0.5 s diastole). SMC number was determined on days 1, 3, 5, 7 and 9. SMC exposed to pressure were more elongated and displayed a significant increase in cell number by day 5 which persisted until day 9. Lactate dehydrogenase (LDH) in the conditioned media, an index of cytotoxicity, was not different between the groups at each time point. There was also no difference in pH or pCO2of the media of SMC in any group. This is the first report of the effects of increased static and pulsatile pressure on SMC in vitro and indicates an increased proliferative rate. We hypothesize that the systemic pressure that the blood vessel is exposed to in vivo may have a significant regulatory influence on the phenotype of the smooth muscle cells which may affect the SMC response to injury or stimuli.