Development of a novel alginate-polyvinyl alcohol-hydroxyapatite hydrogel for 3D bioprinting bone tissue engineered scaffolds
Development of a novel alginate-polyvinyl alcohol-hydroxyapatite hydrogel for 3D bioprinting bone tissue engineered scaffolds
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DOI：
10.1002/jbm.a.36036
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发表时间：
2017-05-01
影响因子：
4.9
通讯作者：
Wei, Mei
中科院分区：
文献类型：
--
作者：
Bendtsen, Stephanie T.;Quinnell, Sean P.;Wei, Mei
作为个性化组织替代品的三维打印生物材料具有促进和增强缺陷组织区域再生的能力。3D打印用于骨组织工程的挑战仍然是选择具有最佳流变性能的材料进行打印，以及生物相容性和均匀细胞结合的能力。水凝胶生物材料可以提供足够的可打印性，允许细胞包封和生物打印具有均匀细胞分布的支架。在这项研究中，一种新的海藻酸-聚乙烯醇(PVA)-羟基磷灰石（HA）水凝胶配方具有最佳的流变学特性，用于将小鼠颅骨3T3-E1 （MC3T3）细胞3D生物打印成高形状保真度的支架。进行了系统的调查，以确定配方中不同浓度的海藻酸盐、磷酸盐、钙和PVA-HA悬浮液对水凝胶粘度的影响，从而确定水凝胶的可印刷性。透明质酸是天然骨中的主要矿物质成分，由于其优异的骨导电性，被加入到水凝胶配方中，创造了良好的骨形成环境。在α - mem细胞培养基中的降解研究表明，3D打印的藻酸盐- pva - ha支架在14天内保持完好。通过完成3D打印过程，MC3T3细胞在最佳水凝胶配方中均匀分布和包裹，并表现出较高的活力。因此，这种新型的、具有骨传导性的、可生物降解的海藻酸- pva - ha配方的发展及其3D生物打印组织工程支架的能力使其成为治疗个性化骨缺陷的有希望的候选材料。(C) 2017 Wiley期刊公司
Three-dimensional printed biomaterials used as personalized tissue substitutes have the ability to promote and enhance regeneration in areas of defected tissue. The challenge with 3D printing for bone tissue engineering remains the selection of a material with optimal rheological properties for printing in addition to biocompatibility and capacity for uniform cell incorporation. Hydrogel biomaterials may provide sufficient printability to allow cell encapsulation and bioprinting of scaffolds with uniform cell distribution. In this study, a novel alginate-polyvinyl alcohol (PVA)-hydroxyapatite (HA) hydrogel formulation with optimal rheological properties for 3D bioprinting of mouse calvaria 3T3-E1 (MC3T3) cells into scaffolds of high shape fidelity has been developed. A systematic investigation was conducted to determine the effect of varying concentrations of alginate, phosphate, calcium, and the PVA-HA suspension in the formulation on the resulting viscosity and thus printability of the hydrogel. HA, the main mineral component in natural bone, was incorporated into the hydrogel formulation to create a favorable bone-forming environment due to its excellent osteoconductivity. Degradation studies in alpha-MEM cell culture media showed that the 3D printed alginate-PVA-HA scaffolds remained in-tact for 14 days. MC3T3 cells were well distributed and encapsulated throughout the optimal hydrogel formulation and expressed high viability through the completion of the 3D printing process. Thus, the development of this novel, osteoconductive, biodegradable, alginate-PVA-HA formulation and its ability to 3D bioprint tissue engineered scaffolds make it a promising candidate for treating personalized bone defects. (C) 2017 Wiley Periodicals, Inc.