Study on the Allelopathy of Alfalfa (Medicago sativa L.): II. Isolation and identification of allelopathic substances in alfalfa
Study on the Allelopathy of Alfalfa (Medicago sativa L.): II. Isolation and identification of allelopathic substances in alfalfa
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苜蓿（Medicago sativa L.）化感作用的研究：II．
DOI：
10.1626/jcs.63.278
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发表时间：
1994
影响因子：
--
通讯作者：
S. Kosemura
中科院分区：
文献类型：
--
作者：
K. Nakahisa;E. Tsuzuki;H. Terao;S. Kosemura
从苜蓿芽的甲醇提取物中纯化苜蓿植物（Medicago sativa L.）中的化感物质。将甲醇提取物分级为酸性级分，并通过薄层色谱（TLC）将样品分为10个级分。 Rf值为0.5～0.6和0.6～0.7的物质对萝卜幼苗的生长有抑制作用。用蒸馏水洗脱这些级分，并通过高效液相色谱（HPLC）分析洗脱液。在色谱图上检测到六个峰。萝卜幼苗的生长在三个峰上均受到抑制。在这些峰中，使用 HPLC、质谱仪、IR 和 1NMR 鉴定了两个峰，如下：峰 b，阿魏酸和峰 f，水杨酸。这些物质浓度较低时，苜蓿和萝卜幼苗的生长受到抑制。
Allelopathic substances in alfalfa plants (Medicago sativa L.) were purified from methanol extracts of alfalfa shoots. The methanol extracts were fractionated into an acidic fraction, and the sample was divided into 10 fractions by thinlayer chromatography (TLC). Growth of radish seedlings was inhibited by the substances obtained from Rf values of 0.5∼0.6 and 0.6∼0.7. These fractions were eluted with distilled water, and the eluates were analyzed by high-performance liquid chromatography (HPLC). Six peaks were detected on the chromatogram. Growth of radish seedlings was inhibited on three peaks. Of these peaks, two were identified using HPLC, a mass-spectrometer, IR, and 1NMR as follows : peak b, ferulic acid and peak f, salicylic acid. The growth of alfalfa and radish seedlings was inhibited at lower concentrations of these substances.