Establishment of bipotent progenitor cell clone from rat skeletal muscle
Establishment of bipotent progenitor cell clone from rat skeletal muscle
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DOI：
10.1111/j.1740-0929.2011.00907.x
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发表时间：
2011-01-01
影响因子：
2
通讯作者：
Nishihara, Masugi
中科院分区：
文献类型：
--
作者：
Murakami, Yousuke;Yada, Erica;Nishihara, Masugi
本研究从大鼠骨骼肌中分离、克隆成脂祖细胞并鉴定其生物学特性。在获得的10个克隆中，进一步表征了最高度成脂祖细胞2G 11细胞。除了它们的成脂性，2G 11细胞保留成肌潜能，如当与成肌细胞共培养时通过形成多核肌管所揭示的。2G 11细胞对碱性成纤维细胞生长因子对脂肪形成的抑制作用具有抗性，而广泛使用的前脂肪形成细胞系3 T3-L1细胞的脂肪形成几乎完全被相同的处理抑制。体内移植实验表明，2G 11细胞能够在体内同时具有成脂性和成肌性。这些结果表明在大鼠骨骼肌中存在双能祖细胞，并表明这些细胞可能有助于骨骼肌中异位脂肪的形成。
The present study describes the isolation, cloning and characterization of adipogenic progenitor cells from rat skeletal muscle. Among the obtained 10 clones, the most highly adipogenic progenitor, 2G11 cells, were further characterized. In addition to their adipogenicity, 2G11 cells retain myogenic potential as revealed by formation of multinucleated myotubes when co-cultured with myoblasts. 2G11 cells were resistant to an inhibitory effect of basic fibroblast growth factor on adipogenesis, while adipogenesis of widely used preadipogenic cell line, 3T3-L1 cells, was suppressed almost completely by the same treatment. In vivo transplantation experiments revealed that 2G11 cells are able to possess both adipogenicity and myogenicity in vivo. These results indicate the presence of bipotent progenitor cells in rat skeletal muscle, and suggest that such cells may contribute to ectopic fat formation in skeletal muscle.