MicroCT liver contrast agent enhancement over time, dose, and mouse strain
MicroCT liver contrast agent enhancement over time, dose, and mouse strain
复制标题
DOI：
10.1007/s11307-007-0128-x
复制
发表时间：
2008-03-01
影响因子：
3.1
通讯作者：
Stout, David B.
中科院分区：
文献类型：
--
作者：
Suckow, Chris E.;Stout, David B.
目的：本研究的目的是研究Fenestra肝造影剂（LC）在Nude、C57和严重联合免疫缺陷（SCID）小鼠中摄取的品系差异。此外，我们旨在确定最佳剂量，并确定Fenestra LC是否对2-脱氧-2[F-18]氟-d -葡萄糖（FDG）值有正电子发射断层扫描（PET）衰减效应。方法：裸鼠、C57小鼠和SCID小鼠分别以5.0、7.5、10.0和15.0 ml/kg的剂量通过尾静脉注射造影剂，并在注射后2小时使用微计算机断层扫描（microCT）成像，然后每天扫描，持续7天。小鼠用FDG、加或不加造影剂进行显微pet /CT成像。结果：不同小鼠品系间对比有显著差异。SCID小鼠脾脏对造影剂的摄取明显高于其他品系，C57小鼠肝脏对造影剂的摄取明显高于其他品系。在所有菌株中，脾脏表现出明显高于肝脏的摄取和造影剂持续时间。对比剂给药后，只有心脏显示FDG摄取的显著衰减。结论：观察了不同菌株对Fenestra LC摄取和信号持续时间的差异。当剂量为7.5 ml/kg时，该造影剂对成像的有效时间为1天，而当剂量为15 ml/kg时，可使用长达1周。对于大多数组织，Fenestra LC在15 ml/kg时似乎不会减弱FDG的摄取；因此，它可以与微pet成像研究结合使用。
Purpose: The aim of this study was to examine strain differences in the uptake of Fenestra liver contrast agent (LC) in Nude, C57, and severe-combined immunodeficient (SCID) mice. In addition, we aimed to determine optimum dosing and to determine if there are positron emission tomography (PET)-attenuation effects on 2-deoxy-2[F-18]fluoro-D-glucose (FDG) values due to Fenestra LC.Procedures: Nude, C57, and SCID mice were injected via tail vein at 5.0, 7.5, 10.0, and 15.0 ml/kg with contrast agent and imaged using micro computed tomography (microCT) 2 h after uptake and then daily up to 7 days. Mice were imaged by microPET/CT with FDG, with or without contrast agent.Results: Significant variations in contrast were observed between mouse strains. SCID mice had significantly more spleen uptake of contrast than the other strains, and C57 mice had significantly more liver uptake of contrast than other strains. Across all strains, the spleen showed significantly higher uptake and duration of contrast than liver. Only the heart showed a significant attenuation of FDG uptake following contrast administration.Conclusions: Strain-specific variations in Fenestra LC uptake and signal duration were observed. At 7.5 ml/kg, this contrast agent is effective for imaging for 1 day, whereas at 15 ml/kg, it can be used up to 1 week. Fenestra LC does not appear to attenuate FDG uptake at 15 ml/kg for most tissues; therefore, it can be used in conjunction with microPET imaging studies.