A CYSTIC-FIBROSIS PANCREATIC ADENOCARCINOMA CELL-LINE
A CYSTIC-FIBROSIS PANCREATIC ADENOCARCINOMA CELL-LINE
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DOI：
10.1073/pnas.87.10.4012
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发表时间：
1990-05-01
影响因子：
11.1
通讯作者：
FRIZZELL, RA
中科院分区：
文献类型：
--
作者：
SCHOUMACHER, RA;RAM, J;FRIZZELL, RA
我们从囊性纤维化（CF）患者中建立了胰腺癌细胞系（CFPAC-1），并评估了其一些特性。细胞呈上皮形态，表达胰管细胞特有的细胞角蛋白和癌胚抗原。cAMP和cAMP依赖性蛋白激酶的基础和刺激水平以及CFPAC-1中单个Cl-通道的生物物理性质与气道和汗腺原代培养物和Cl-分泌上皮细胞系的相似。阴离子转运和单氯离子通道活性的刺激钙离子载体，但不是由毛喉素，cAMP类似物，或磷酸二酯酶抑制剂。这些细胞表达CF基因，并表现出最常见的CF突变，三个核苷酸的缺失导致苯丙氨酸-508缺失。这些特性在> 80代（24个月）中一直是稳定的，表明CFPAC-1可以用作显示CF缺陷的连续细胞系。
We established a pancreatic adenocarcinoma cell line (CFPAC-1) from a patient cystic fibrosis (CF) and assessed some of its properties. The cells show epithelial morphology and expressed cytokeratin and oncofetal antigens characteristic of pancreatic duct cells. Basal and stimulated levels of cAMP and cAMP-dependent protein kinase and the biophysical properties of single Cl- channels in CFPAC-1 are similar to those of airway and sweat gland primary cultures and Cl--secreting epithelial cell lines. Anion transport and single Cl- channel activity was stimulated by Ca2+ ionophores but not by forskolin, cAMP analogs, or phosphodiesterase inhibitors. The cells express the CF gene and manifest the most common CF mutation, deletion of three nucleotides resulting in a phenylalanine-508 deletion. These properties have been stable through > 80 passages (24 months), suggesting that CFPAC-1 can serve as a continuous cell line that displays the CF defect.