OXIDATION OF NONPHENOLIC SUBSTRATES - AN EXPANDED ROLE FOR LACCASE IN LIGNIN BIODEGRADATION
OXIDATION OF NONPHENOLIC SUBSTRATES - AN EXPANDED ROLE FOR LACCASE IN LIGNIN BIODEGRADATION
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DOI：
10.1016/0014-5793(90)80298-w
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发表时间：
1990-07-02
期刊：
影响因子：
3.5
通讯作者：
PAICE, MG
中科院分区：
文献类型：
--
作者：
BOURBONNAIS, R;PAICE, MG
在底物如Remazol Blue和2，2 '-azinobis（3-ethylbenzthiazoline-6-sulfonate）（ABTS）的存在下，漆酶Coriolus（Trametes）versicolor也可以氧化非酚木质素模型化合物。藜芦醇（I）和1-（3，4-二甲氧基苯基）-2-（2-甲氧基苯氧基）-1，3-丙二醇（III）在漆酶和介体作用下被氧化生成α-羰基衍生物。在ABTS存在下，漆酶裂解β-1木质素模型二聚体1-（3，4-二甲氧基苯基）-2-苯氧基-1，2-乙二醇（II），得到藜芦醛和苯甲醛。在这些观察的基础上，我们提出，漆酶是能够氧化木质素的酚和非酚部分，但后者是依赖于主要漆酶底物的共存。
In the presence of substrates such as Remazol Blue and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate) (ABTS), laccasesCoriolus (Trametes)versicolorcan also oxidize non-phenolic lignin model compounds. Veratryl alcohol (I) and 1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)-propane-1, 3-diol (III) were oxidized by laccase and mediator to give the α-carbonyl derivatives. The β-1 lignin model dimer, 1-(3,4-dimethoxyphenyl)-2-phenoxy-ethane-1, 2-diol(II) was cleaved by laccase in the presence of ABTS to give veratraldehyde and benzaldehyde. On the basis of these observations, we propose that laccase is capable of oxidizing both phenolic and non-phenolic moieties of lignin but that the latter is dependent on the co-presence of primary laccase substrates.