Enzymatic degradation of β-chitin:: susceptibility and the influence of deacetylation
Enzymatic degradation of β-chitin:: susceptibility and the influence of deacetylation
复制标题
DOI：
10.1016/s0144-8617(99)00127-7
复制
发表时间：
2000-05-01
影响因子：
11.2
通讯作者：
Nishiyama, Y
中科院分区：
文献类型：
--
作者：
Kurita, K;Kaji, Y;Nishiyama, Y
研究了β-甲壳素对溶菌酶的敏感性以及脱乙酰基对酶促降解行为的影响。由于弱的分子间力，β-甲壳素比α-甲壳素更容易降解。在温和条件下制备部分脱乙酰基的β-几丁质，并对其进行溶菌酶处理。脱乙酰化程度对降解率有显著影响，在脱乙酰化度约为50%时降解率达到最大。然后速率降低，并且脱乙酰度为0.97的衍生的壳聚糖根本不降解。因此，降解性的精细控制成为可能，表明β-甲壳素作为具有低毒性的生物可降解特种聚合物的高潜力。(C)2000爱思唯尔科技有限公司版权所有。
Susceptibility of beta-chitin to lysozyme and the influence of deacetylation on the enzymatic degradation behavior have been elucidated. beta-Chitin was degraded much more readily than alpha-chitin due to the weak intermolecular forces. Partially deacetylated beta-chitins were prepared under mild conditions and subjected to lysozyme treatment. The degradation rate proved to be affected markedly by the extent of deacetylation and showed a maximum at about 50% deacetylation. The rate then decreased, and the derived chitosan with a degree of deacetylation of 0.97 was not degraded at all. Fine control of degradability thus became possible, indicating a high potential of beta-chitin as a biodegradable specialty polymer with low toxicity. (C) 2000 Elsevier Science Ltd. All rights reserved.