DEVELOPMENTAL-CHANGES IN INTRACELLULAR CALCIUM REGULATION IN RAT CEREBRAL-CORTEX DURING HYPOXIA
DEVELOPMENTAL-CHANGES IN INTRACELLULAR CALCIUM REGULATION IN RAT CEREBRAL-CORTEX DURING HYPOXIA
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DOI：
10.1038/jcbfm.1993.103
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发表时间：
1993-09-01
影响因子：
6.3
通讯作者：
HANSEN, BM
中科院分区：
文献类型：
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作者：
BICKLER, PE;GALLEGO, SM;HANSEN, BM
在生命的最初几周，由短暂缺氧引起的中枢神经系统损伤大大增加。为了探讨这种变化的可能原因，缺氧或兴奋性神经递质谷氨酸的应用对细胞内钙（[Ca 2 +]i）和ATP的影响进行了研究，在大鼠大脑皮层脑片。[Ca2+]i用指示剂Fura-2荧光测定。缺氧（95%N2/5%CO2）或100 μ M氰化钠可使大鼠脑片[Ca 2 +]i和ATP耗竭逐渐升高
During the first weeks of life, injury to the central nervous system caused by brief periods of oxygen deprivation greatly increases. To investigate possible causes for this change, the effects of hypoxia or application of the excitatory neurotransmitter glutamate on intracellular calcium ([Ca2+]i) and ATP were studied in rat cerebrocortical brain slices. [Ca2+]i was measured fluorometrically with the indicator Fura-2. Hypoxia (95% N2/5% CO2) or 100 muM sodium cyanide produced gradual elevations in [Ca2+]i and ATP depletion in slices from rats