Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn's disease using terminal restriction fragment length polymorphism analysis
Comparison of the fecal microbiota profiles between ulcerative colitis and Crohn's disease using terminal restriction fragment length polymorphism analysis
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DOI：
10.1007/s00535-010-0368-4
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发表时间：
2011-04-01
影响因子：
6.3
通讯作者：
Fujiyama, Yoshihide
中科院分区：
文献类型：
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作者：
Andoh, Akira;Imaeda, Hirotsugu;Fujiyama, Yoshihide
末端限制性片段长度多态性（T-RFLP）分析是评估微生物群落多样性的有力工具。本研究对31例溃疡性结肠炎（UC）患者、31例克罗恩病（CD）患者和30例健康人的粪便微生物群进行了T-RFLP分析。对粪便样品的16 SrRNA基因进行PCR扩增，用BslI酶切，测定T-RF长度，将粪便微生物群落分为5个类群。30例健康个体中的28例和18例非活动性UC患者中的17例被分类为I、II和III类，但这些聚类包括少数活动性UC和非活动性/活动性CD患者。相比之下，13名活动性UC患者中的8名和大多数CD患者（16名非活动性CD患者中的12名和15名活动性CD患者中的11名）被包括在簇IV和簇V中。基于BsII消化的T-RFLP数据库，细菌显示活动性UC和非活动性/活动性CD患者中梭菌家族的显著减少。相反，类杆菌在CD患者中显著增加。活动期UC和活动期CD患者粪便微生物菌群差异无统计学意义。IBD患者粪便微生物菌群与健康人不同。非活动性UC患者的肠道微生物群倾向于更接近健康个体的肠道微生物群，这表明粪便微生物群在UC和CD的病理生理学中的不同作用。
Terminal restriction fragment length polymorphism (T-RFLP) analysis is a powerful tool to assess the diversity of a microbial community. In this study, we performed T-RFLP analysis of the fecal microbiota from patients with ulcerative colitis (UC) and those with Crohn's disease (CD).Thirty-one patients with UC, 31 patients with CD, and 30 healthy individuals were enrolled. The polymerase chain reaction (PCR) products obtained from the 16S rRNA genes of fecal samples were digested with BslI, and T-RF lengths were determined.The fecal microbial communities were classified into 5 clusters. Twenty-eight of the 30 healthy individuals and 17 of the 18 patients with inactive UC were classified into clusters I, II, and III, but these clusters included a small number of patients with active UC and inactive/active CD. In contrast, 8 of the 13 patients with active UC and the majority of CD patients (12 of the 16 patients with inactive CD, and 11 of the 15 patients with active CD) were included in clusters IV and V. Based on the BslI-digested T-RFLP database, the bacteria showed a significant decrease in the Clostridium family in patients with active UC and inactive/active CD. In contrast, Bacteroides were significantly increased in CD patients. No significant differences were observed between patients with active UC and those with active CD.The fecal microbial communities of IBD patients were different from those of healthy individuals. The gut microbiota of patients with inactive UC tended to be closer to that of healthy individuals, suggesting different roles for the fecal microbiota in the pathophysiology of UC and CD.