Dendrimer-based micelles as cyto-compatible nanocarriers
Dendrimer-based micelles as cyto-compatible nanocarriers
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DOI：
10.1039/c9nj02612f
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发表时间：
2019-08-14
影响因子：
3.3
通讯作者：
Sharma, Sunil K.
中科院分区：
文献类型：
--
作者：
Parshad, Badri;Yadav, Preeti;Sharma, Sunil K.
已经使用生物相容性材料如甘油、葡萄糖、间苯三酚和各种尺寸的烷基链合成了一系列树枝状结构，以使它们具有水溶性并获得适当的疏水-疏水平衡，从而使它们形成纳米尺寸的聚集体，用于封装和递送用于生物医学应用的药物/染料。使用荧光测量、动态光散射（DLS）、透射电子显微镜（TEM）、通过共聚焦激光扫描显微镜（cLSM）的活细胞成像和流式细胞术研究了这些树突状结构的聚集行为以及它们的细胞毒性和它们的细胞摄取。聚集研究表明，树枝状大分子在低微摩尔浓度的水溶液中能够很好地自组装，形成均匀分布的胶束聚集体。装载到树枝状聚合物中的尼罗红的有效细胞摄取，以及树枝状聚合物的非常低的毒性，表明它们作为药物和成像剂的载体的潜力。
A series of dendritic architectures have been synthesized using biocompatible materials such as glycerol, glucose, phloroglucinol, and alkyl chains of various sizes to give them aqueous solubility and to attain proper hydrophilic-hydrophobic balance, allowing them to form nano-sized aggregates for the encapsulation and delivery of drugs/dyes for biomedical applications. The aggregation behavior of these dendritic architectures, as well as their cytotoxicity and their cellular uptake, was studied using fluorescence measurements, dynamic light scattering (DLS), transmission electron microscopy (TEM), live cell imaging by confocal laser scanning microscopy (cLSM), and flow cytometry. The aggregation study has shown that the dendrimers proficiently self-assemble in aqueous solution with low micromolar concentration and form uniformly spread micellar aggregates. The effective cellular uptake of Nile red loaded into the dendrimers, as well as the very low toxicity of the dendrimers, indicates their potential as carriers for drugs and imaging agents.