Comparison of two phosphoproteins in chicken bone and their similarities to the mammalian bone proteins, osteopontin and bone sialoprotein II.
Comparison of two phosphoproteins in chicken bone and their similarities to the mammalian bone proteins, osteopontin and bone sialoprotein II.
复制标题
鸡骨中两种磷蛋白的比较及其与哺乳动物骨蛋白骨桥蛋白和骨唾液酸蛋白II的相似性。
DOI：
10.1016/s0006-291x(05)81082-4
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Glimcher,MJ
中科院分区：
文献类型：
--
作者：
Gotoh,Y;Pierschbacher,MD;Grzesiak,JJ;Gerstenfeld,L;Glimcher,MJ
Two phosphorylated proteins of ∼66 kDa and ∼60 kDa mass with different DEAE-Sephacel elution patterns were isolated from chicken bone and were shown to be genetically distinct by both biochemical and immunological analysis. A tryptic peptide from the 60 kDa protein was identified that was similar to a sequence of the rat bone sialoprotein II. Both proteins showed RGD inhibited cell-attachment with the MG-63 osteosarcoma cell, and the ∼66 kDa phosphoprotein appeared to promote cell adhesion better than human vitronectin. The two phosphoproteins appear to share functional and biochemical characteristics and to be homologous to the mammalian bone phosphoproteins, osteopontin and bone sialoprotein II.
