Differentiation of an ameloblast-lineage cell line (ALC) is induced by Sonic hedgehog signaling
Differentiation of an ameloblast-lineage cell line (ALC) is induced by Sonic hedgehog signaling
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DOI：
10.1016/j.bbrc.2006.12.053
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发表时间：
2007-02-09
影响因子：
3.1
通讯作者：
Suda, Hideaki
中科院分区：
文献类型：
--
作者：
Takahashi, Satomi;Kawashima, Nobuyuki;Suda, Hideaki
Sonic hedgehog（Shh）是胚胎发生和器官发生所必需的分子之一，在牙釉质结中有强表达，由于存在必需的分泌分子，牙釉质结是牙发生的信号中心。在这里，我们研究了Shh对成釉细胞分化的影响，使用小鼠成釉细胞系（ALC）。在Shh的存在下，釉原蛋白和成釉蛋白的表达上调，其启动子活性也升高。将Shh处理的ALC皮下植入C57/B16 J小鼠背部，引起釉原蛋白和成釉蛋白的表达增加。Gli 1是Shh信号传导中的主要转录因子，其强制表达上调了釉原蛋白和成釉蛋白，而Gli 1特异性RNAi阻断了它们的表达。这些结果表明，Shh直接促进ALC分化，Gli 1可能负责Shh的信号介导。(c)2006爱思唯尔公司All rights reserved.
Sonic hedgehog (Shh), one of the essential molecules for embryogenesis and organogenesis, is strongly expressed in the enamel knot, which represents the signaling center for odontogenesis due to the presence of essential secretory molecules. Here, we investigated the effects of Shh on ameloblast differentiation using a mouse ameloblast-lineage cell line (ALC). In the presence of Shh, the expression of amelogenin and ameloblastin was up-regulated, and their promoter activities were also elevated. Subcutaneous implantation of Shh-treated ALC into the dorsa of C57/B16J mouse caused increased expression of amelogenin and ameloblastin. Forced expression of Gli1, a major transcription factor in Shh signaling, up-regulated both amelogenin and ameloblastin, while Gli1-specific RNAi blocked their expression. These results suggest that Shh directly promotes ALC differentiation, and that Gli1 may be responsible for the signal mediation of Shh. (c) 2006 Elsevier Inc. All rights reserved.