Fluorescent hydrogels for embryoid body formation and osteogenic differentiation of embryonic stem cells.
Fluorescent hydrogels for embryoid body formation and osteogenic differentiation of embryonic stem cells.
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DOI：
10.1021/acsami.5b02368
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发表时间：
2015-05
影响因子：
9.5
通讯作者：
N. Z. Zur Nieden;Cassandra C. Turgman;Xuye Lang;Jillian M. Larsen;Joseph Granelli;Yu-Jer Hwang;Julia G. Lyubovitsky
中科院分区：
文献类型：
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作者：
N. Z. Zur Nieden;Cassandra C. Turgman;Xuye Lang;Jillian M. Larsen;Joseph Granelli;Yu-Jer Hwang;Julia G. Lyubovitsky
基质力学（例如，微环境的刚度和地形）可能对驱动正常形态发生和组织发育至关重要。因此，由水凝胶施加的底物力学已被用于指导干细胞的谱系分化和驱动干性。在这项工作中，我们通过甘油醛交联对明胶水凝胶进行化学修饰，使其适合细胞培养。经过修饰的水凝胶被证明是胚胎干细胞成骨的理想材料，最初为胚状体的形成提供了一个柔软的非粘附表面。它们随后在培养过程中降解，在成骨细胞分化过程中提供更硬的表面。合成的凝胶具有高荧光性，相对容易制备，并且可以潜在地帮助克服三维细胞培养过程中细胞微环境成像变化的挑战。利用这些材料可以开发出更复杂的组织工程产品和合理的治疗策略。
Substrate mechanics (e.g., stiffness and topography of the microenvironment) are likely critical for driving normal morphogenesis and tissue development. As such, substrate mechanics imposed by hydrogels have been exploited to guide the lineage differentiation of stem cells and to drive stemness. In this work, we chemically modified gelatin hydrogels through glyceraldehyde cross-linking to render them suitable for cell culture. The modified hydrogels proved to be ideal for embryonic stem cell osteogenesis, initially providing a soft nonadhesive surface for the formation of embryoid bodies. They subsequently degraded in culture to afford a harder surface during osteoblast differentiation. The gels synthesized are highly fluorescent, relatively easy to prepare, and can potentially aid in overcoming the challenge of imaging changes to the microenvironments of cells during three-dimensional cell culture. Exploiting these materials could lead to the development of tissue-engineered products of increased complexity and rational treatment strategies.